Release of Soybean Isoflavones by Using a β-Glucosidase from Alicyclobacillus herbarius

Delgado, Lidia; Heckmann, Christian M.; Di Pisa, Flavio; Gourlay, Louise; Paradisi, Francesca

Abstract

β-Glucosidases are used in the food industry to hydrolyse glycosidic bonds in complex sugars, with enzymes sourced from extremophiles better able to tolerate the process conditions. In this work, a novel β-glycosidase from the acidophilic organism Alicyclobacillus herbarius was cloned and heterologously expressed in Escherichia coli BL21(DE3). AheGH1 was stable over a broad range of pH values (5–11) and temperatures (4–55 °C). The enzyme exhibited excellent tolerance to fructose and good tolerance to glucose, retaining 65 % activity in the presence of 10 % (w/v) glucose. It also tolerated organic solvents, some of which appeared to have a stimulating effect, in particular ethanol with a 1.7-fold increase in activity at 10 % (v/v). The enzyme was then applied for the cleavage of isoflavone from isoflavone glucosides in an ethanolic extract of soy flour, to produce soy isoflavones, which constitute a valuable food supplement, full conversion was achieved within 15 min at 30 °C.

Citation

Delgado, L., Heckmann, C. M., Di Pisa, F., Gourlay, L., & Paradisi, F. (2021). Release of Soybean Isoflavones by Using a β-Glucosidase from Alicyclobacillus herbarius. ChemBioChem, 22(7), 1223-1231. https://doi.org/10.1002/cbic.202000688

• Journal Article Type: Article
• Acceptance Date: Nov 25, 2020
• Online Publication Date: Dec 30, 2020
• Publication Date: Apr 6, 2021
• Deposit Date: Dec 16, 2020
• Publicly Available Date: Dec 30, 2020
• Journal: ChemBioChem
• Print ISSN: 1439-4227
• Electronic ISSN: 1439-7633
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 22
• Issue: 7
• Pages: 1223-1231
• DOI: https://doi.org/10.1002/cbic.202000688
• Keywords: Organic Chemistry; Molecular Medicine; Biochemistry; Molecular Biology
• Public URL: https://nottingham-repository.worktribe.com/output/5152823
• Publisher URL: https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cbic.202000688