Minimal models for RNA simulations

Thirumalai, D.; Hori, Naoto; Nguyen, Hung T.

Abstract

The increasing importance of RNA as a prime player in biology can hardly be overstated. Problems in RNA, such as folding and RNA–RNA interactions that drive phase separation, require cations. Because experiments alone cannot reveal the dynamics of cation-RNA interactions, well calibrated theory and computations are needed to predict how ions control the behavior of RNA. The perspective describes the development and use of coarse-grained models at different resolutions. We focus on single- and three-interaction site models, in which electrostatic interactions are treated using a combination of explicit and implicit representations. Applications to the folding of ribozymes and riboswitches are discussed, with emphasis on the role of monovalent and divalent cations. We also discuss phase separation in low-complexity sequences. Challenges in the simulation of complex problems such as ribosome assembly and RNA chaperones, requiring developments of models for RNA-protein interactions, are pointed out.

Citation

Thirumalai, D., Hori, N., & Nguyen, H. T. (2025). Minimal models for RNA simulations. Current Opinion in Structural Biology, 93, Article 103107. https://doi.org/10.1016/j.sbi.2025.103107

• Journal Article Type: Article
• Acceptance Date: Jun 9, 2025
• Online Publication Date: Jul 5, 2025
• Publication Date: 2025-08
• Deposit Date: Jul 5, 2025
• Publicly Available Date: Jul 8, 2025
• Journal: Current Opinion in Structural Biology
• Print ISSN: 0959-440X
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 93
• Article Number: 103107
• DOI: https://doi.org/10.1016/j.sbi.2025.103107
• Public URL: https://nottingham-repository.worktribe.com/output/51190501
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0959440X25001253