An improved oil palm genome assembly as a valuable resource for crop improvement and comparative genomics in the Arecoideae subfamily

Ong, Ai Ling; Teh, Chee Keng; Mayes, Sean; Massawe, Festo; Appleton, David Ross; Kulaveerasingam, Harikrishna

Abstract

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Oil palm (Elaeis guineensis Jacq.) is the most traded crop among the economically important palm species. Here, we report an extended version genome of E. guineensis that is 1.2 Gb in length, an improvement of the physical genome coverage to 79% from the previous 43%. The improvement was made by assigning an additional 1968 originally unplaced scaffolds that were available publicly into the physical genome. By integrating three ultra-dense linkage maps and using them to place genomic scaffolds, the 16 pseudomolecules were extended. As we show, the improved genome has enhanced the mapping resolution for genome-wide association studies (GWAS) and permitted further identification of candidate genes/protein-coding regions (CDSs) and any non-coding RNA that may be associated with them for further studies. We then employed the new physical map in a comparative genomics study against two other agriculturally and economically important palm species—date palm (Phoenix dactylifera L.) and coconut palm (Cocos nucifera L.)—confirming the high level of conserved synteny among these palm species. We also used the improved oil palm genome assembly version as a palm genome reference to extend the date palm physical map. The improved genome of oil palm will enable molecular breeding approaches to expedite crop improvement, especially in the largest subfamily of Arecoideae, which consists of 107 species belonging to Arecaceae.

Citation

Ong, A. L., Teh, C. K., Mayes, S., Massawe, F., Appleton, D. R., & Kulaveerasingam, H. (2020). An improved oil palm genome assembly as a valuable resource for crop improvement and comparative genomics in the Arecoideae subfamily. Plants, 9(11), Article 1476. https://doi.org/10.3390/plants9111476

• Journal Article Type: Article
• Acceptance Date: Oct 28, 2020
• Online Publication Date: Nov 3, 2020
• Publication Date: Nov 1, 2020
• Deposit Date: Nov 3, 2020
• Publicly Available Date: Nov 4, 2020
• Journal: Plants
• Electronic ISSN: 2223-7747
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 9
• Issue: 11
• Article Number: 1476
• DOI: https://doi.org/10.3390/plants9111476
• Keywords: Elaeis guineensis; high-density linkage maps; physical maps; comparative genomics; genome-enabled breeding; Arecoideae; pseudomolecules
• Public URL: https://nottingham-repository.worktribe.com/output/5013750
• Publisher URL: https://www.mdpi.com/2223-7747/9/11/1476