Continuous preparation of flavour‐active acetate esters by direct biocatalytic esterification

Perdomo, Igor Chiarelli; Contente, Martina Letizia; Pinto, Andrea; Romano, Diego; Fernandes, Pedro; Molinari, Francesco

Abstract

Mycelium-bound lipase(s) from Aspergillus oryzae catalysed direct esterification of isoamyl alcohol and cinnamyl alcohol with acetic acid in organic solvent, showing high stability towards substrates and products. Water produced during the esterification did not significantly affect the equilibrium of the reaction, allowing for high conversions. These features were exploited for preparing flavour-active acetate esters (isoamyl and cinnamyl acetate) in batch and continuous systems. A continuous stirred tank membrane reactor (CST-MR) was developed securing good reactor productivity and high biocatalyst stability.

Citation

Perdomo, I. C., Contente, M. L., Pinto, A., Romano, D., Fernandes, P., & Molinari, F. (2020). Continuous preparation of flavour‐active acetate esters by direct biocatalytic esterification. Flavour and Fragrance Journal, 35(2), 190-196. https://doi.org/10.1002/ffj.3552

• Journal Article Type: Article
• Acceptance Date: Oct 19, 2019
• Online Publication Date: Nov 6, 2019
• Publication Date: 2020-03
• Deposit Date: Sep 8, 2020
• Publicly Available Date: Jan 26, 2021
• Journal: Flavour and Fragrance Journal
• Print ISSN: 0882-5734
• Electronic ISSN: 1099-1026
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 35
• Issue: 2
• Pages: 190-196
• DOI: https://doi.org/10.1002/ffj.3552
• Keywords: Food Science; General Chemistry
• Public URL: https://nottingham-repository.worktribe.com/output/4889962
• Publisher URL: https://onlinelibrary.wiley.com/doi/abs/10.1002/ffj.3552
• Additional Information: This is the peer reviewed version of the following article: Chiarelli Perdomo, I, Letizia Contente, M, Pinto, A, Romano, D, Fernandes, P, Molinari, F. Continuous preparation of flavour‐active acetate esters by direct biocatalytic esterification. Flavour Fragr J. 2020; 35: 190– 196, which has been published in final form at https://doi.org/10.1002/ffj.3552. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.