Epidermal Growth Factor Variations in Amniotic Membrane Used for Ex Vivo Tissue Constructs

Gicquel, Jean-Jacques; Dua, Harminder S.; Brodie, Andrew; Mohammed, Imran; Suleman, Hanif; Lazutina, Elena; James, David K.; Hopkinson, Andrew

Abstract

Introduction: The amniotic membrane (AM) is used for engineering ex vivo tissue constructs used in ocular surface reconstruction. Epidermal growth factor (EGF) content of the AM is believed to play a key role in supporting corneal epithelial cell expansion on AM. This study investigated EGF content in AM in relation to intra- and inter-donor variations and the effect of processing and preservation (handling).

Methods: Fifteen human AM, both fresh and handled, were analyzed for EGF gene and protein expression by real-time polymerase chain reaction and ELISA, respectively.

Results: EGF gene expression was predominantly seen in the AM epithelium (p 

Citation

Gicquel, J.-J., Dua, H. S., Brodie, A., Mohammed, I., Suleman, H., Lazutina, E., James, D. K., & Hopkinson, A. (2009). Epidermal Growth Factor Variations in Amniotic Membrane Used for Ex Vivo Tissue Constructs. Tissue Engineering Part A, 15(8), 1919-1927. https://doi.org/10.1089/ten.tea.2008.0432

• Journal Article Type: Article
• Online Publication Date: Jan 19, 2009
• Publication Date: 2009-08
• Deposit Date: Jul 21, 2020
• Journal: Tissue Engineering Part A
• Print ISSN: 1937-3341
• Electronic ISSN: 1937-335X
• Publisher: Mary Ann Liebert
• Peer Reviewed: Peer Reviewed
• Volume: 15
• Issue: 8
• Pages: 1919-1927
• DOI: https://doi.org/10.1089/ten.tea.2008.0432
• Public URL: https://nottingham-repository.worktribe.com/output/4781089
• Publisher URL: https://www.liebertpub.com/doi/10.1089/ten.tea.2008.0432