Candidate genes linked to QTL regions associated with fatty acid composition in oil palm

Ting, Ngoot Chin; Mayes, Sean; Massawe, Festo; Sambanthamurthi, Ravigadevi; Chan, Kuang Lim; Sritharan, Kandha; Singh, Rajinder

Abstract

© 2020, Institute of Molecular Biology, Slovak Academy of Sciences. The present study searched for candidate genes in five linkage groups (LGs) - T2, T3, OT4, OT6 and T9 hosting the QTLs associated with iodine value (IV) and fatty acid composition (FAC) in an oil palm interspecific hybrid population. Each of the five LGs was successfully anchored to its corresponding chromosomal segment where, a wider repertoire of candidate genes was identified. This study further revealed a total of 19 candidate genes and four transcription factors involved in biosynthesis of fatty acids, lipids (including triacylglycerol) and acetyl-CoA, glycosylation and degradation of fatty acids. Their possible involvement in regulating the levels of saturation are discussed. In addition, 22 candidate genes located outside the QTL intervals were also identified across the interspecific hybrid genome. A total of 92 SSR markers were developed to tag the presence of these candidate genes and 50 were successfully mapped onto their respective positions on the genome. The data obtained here complements the previous studies, and collectively, these QTL-linked candidate gene markers could help breeders in more precisely selecting palms with the desired FAC.

• Journal Article Type: Article
• Acceptance Date: Jul 3, 2020
• Online Publication Date: Jul 17, 2020
• Publication Date: Jul 17, 2020
• Deposit Date: Jul 20, 2020
• Publicly Available Date: Jul 18, 2021
• Journal: Biologia
• Print ISSN: 0006-3088
• Electronic ISSN: 1336-9563
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 76
• Pages: 267–279
• DOI: https://doi.org/10.2478/s11756-020-00563-2
• Keywords: Plant Science; Animal Science and Zoology; Genetics; Cell Biology; Biochemistry; Ecology, Evolution, Behavior and Systematics; Molecular Biology
• Public URL: https://nottingham-repository.worktribe.com/output/4778208
• Publisher URL: https://link.springer.com/article/10.2478/s11756-020-00563-2
• Additional Information: This is a post-peer-review, pre-copyedit version of an article published in Biologia. The final authenticated version is available online at: http://dx.doi.org/10.2478/s11756-020-00563-2