Remote platelet function testing using P-selectin expression in patients with recent cerebral ischaemia on clopidogrel

Appleton, Jason P.; Richardson, Carla; Dovlatova, Natalia; May, Jane; Sprigg, Nikola; Heptinstall, Stan; Bath, Philip M.

Abstract

Background Antiplatelet agents reduce recurrence after cerebral ischaemia but are not effective in all patients, in part because of treatment resistance. The primary aim was to assess the proportion of patients who are insensitive to clopidogrel. The secondary aim was to assess the association between insensitivity to clopidogrel and recurrent cerebrovascular events.

Methods Following written informed consent, independent patients with a recent noncardioembolic ischaemic stroke or TIA, and taking clopidogrel, were enrolled. Platelet function was assessed with remote measurement of surface expression of P-selectin (CD62P) using commercial kits sensitive to aspirin or clopidogrel. Participants’ general practitioners provided details on recurrent vascular events at least 90 days later. Data are mean (standard deviation) and median [interquartile range]. Resistance was defined as: aspirin median fluorescence (MF) >500 units, clopidogrel MF >860 units. Non-parametric descriptors and tests were used.

Results 63 patients were recruited: mean age 64 (13.7) years, female 47%. At baseline, 59 (95%) patients were taking clopidogrel alone with three (5%) on combined clopidogrel and aspirin. Assessment of platelet surface P-selectin revealed: aspirin test 528 [317, 834], >500 54.8%; clopidogrel test 429 [303, 656], >860 11.3%. No participants on aspirin and clopidogrel showed aspirin resistance. Thirteen (20.6%) patients had a recurrent cerebrovascular event; those with an ischaemic stroke had a non-significantly higher baseline P-selectin using the clopidogrel test as compared with those with no recurrence: 626 [380, 801] vs. 406 [265, 609], p=0.08.

Conclusions Remote measurement of platelet function assessed using the platelet surface expression of P-selectin is feasible. 11% of patients taking clopidogrel showed resistance. No significant associations were noted between clopidogrel resistance and recurrent ischaemic events.

Citation

Appleton, J. P., Richardson, C., Dovlatova, N., May, J., Sprigg, N., Heptinstall, S., & Bath, P. M. (2021). Remote platelet function testing using P-selectin expression in patients with recent cerebral ischaemia on clopidogrel. Stroke and Vascular Neurology, 6(1), 103-108. https://doi.org/10.1136/svn-2020-000346

• Journal Article Type: Article
• Acceptance Date: Jul 14, 2020
• Online Publication Date: Sep 24, 2020
• Publication Date: Mar 25, 2021
• Deposit Date: Jul 15, 2020
• Publicly Available Date: Sep 24, 2020
• Journal: Stroke and Vascular Neurology
• Electronic ISSN: 2059-8696
• Publisher: BMJ Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 6
• Issue: 1
• Article Number: e000346
• Pages: 103-108
• DOI: https://doi.org/10.1136/svn-2020-000346
• Public URL: https://nottingham-repository.worktribe.com/output/4768591
• Publisher URL: https://svn.bmj.com/content/6/1/103