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The role of CAPG in molecular communication between the embryo and the uterine endometrium: Is its function conserved in species with different implantation strategies?

Tinning, Haidee; Taylor, Alysha; Wang, Dapeng; Constantinides, Bede; Sutton, Ruth; Oikonomou, Georgios; Velazquez, Miguel A.; Thompson, Paul; Treumann, Achim; O'Connell, Mary J.; Forde, Niamh

The role of CAPG in molecular communication between the embryo and the uterine endometrium: Is its function conserved in species with different implantation strategies? Thumbnail


Authors

Haidee Tinning

Alysha Taylor

Dapeng Wang

Bede Constantinides

Ruth Sutton

Georgios Oikonomou

Miguel A. Velazquez

Paul Thompson

Achim Treumann

Niamh Forde



Abstract

© 2020. The Authors. The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology During the preimplantation period of pregnancy in eutherian mammals, transcriptional and proteomic changes in the uterine endometrium are required to facilitate receptivity to an implanting blastocyst. These changes are mediated, in part, by proteins produced by the developing conceptus (inner cell mass and extraembryonic membranes). We hypothesized that this common process in early pregnancy in eutheria may be facilitated by highly conserved conceptus‐derived proteins such as macrophage capping protein (CAPG). We propose that CAPG may share functionality in modifying the transcriptome of the endometrial epithelial cells to facilitate receptivity to implantation in species with different implantation strategies. A recombinant bovine form of CAPG (91% sequence identity between bovine and human) was produced and bovine endometrial epithelial (bEECs) and stromal (bESCs) and human endometrial epithelial cells (hEECs) were cultured for 24 hours with and without recombinant bovine CAPG (rbCAPG). RNA sequencing and quantitative real‐time PCR analysis were used to assess the transcriptional response to rbCAPG (Control, vehicle, CAPG 10, 100, 1000 ng/mL: n = 3 biological replicates per treatment per species). Treatment of bEECs with CAPG resulted in alterations in the abundance of 1052 transcripts (629 increased and 423 decreased) compared to vehicle controls. Treatment of hEECs with bovine CAPG increased expression of transcripts previously known to interact with CAPG in different systems (CAPZB, CAPZA2, ADD1, and ADK ) compared with vehicle controls (P [less than] .05). In conclusion, we have demonstrated that CAPG, a highly conserved protein in eutherian mammals, elicits a transcriptional response in the endometrial epithelium in species with different implantation strategies that may contribute to pregnancy success.

Citation

Tinning, H., Taylor, A., Wang, D., Constantinides, B., Sutton, R., Oikonomou, G., …Forde, N. (2020). The role of CAPG in molecular communication between the embryo and the uterine endometrium: Is its function conserved in species with different implantation strategies?. FASEB Journal, 34(8), 11015-11029. https://doi.org/10.1096/fj.202000882rr

Journal Article Type Article
Acceptance Date Jun 9, 2020
Online Publication Date Jul 3, 2020
Publication Date 2020-08
Deposit Date Jul 14, 2020
Publicly Available Date Mar 28, 2024
Journal The FASEB Journal
Print ISSN 0892-6638
Electronic ISSN 1530-6860
Publisher Federation of American Society of Experimental Biology
Peer Reviewed Peer Reviewed
Volume 34
Issue 8
Pages 11015-11029
DOI https://doi.org/10.1096/fj.202000882rr
Keywords Biotechnology; Genetics; Biochemistry; Molecular Biology
Public URL https://nottingham-repository.worktribe.com/output/4764469
Publisher URL https://faseb.onlinelibrary.wiley.com/doi/10.1096/fj.202000882RR

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