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Development and validation of a cost-effective and sensitive bioanalytical HPLC-UV method for determination of lopinavir in rat and human plasma

Qin, Chaolong; Feng, Wanshan; Chu, Yen Ju; Lee, Jong Bong; Berton, Mattia; Bettonte, Sara; Teo, Yeong Yeu; Stocks, Michael J.; Fischer, Peter M.; Gershkovich, Pavel

Development and validation of a cost-effective and sensitive bioanalytical HPLC-UV method for determination of lopinavir in rat and human plasma Thumbnail


Authors

Chaolong Qin

Wanshan Feng

Yen Ju Chu

Jong Bong Lee

Mattia Berton

Sara Bettonte

Yeong Yeu Teo

MICHAEL STOCKS MICHAEL.STOCKS@NOTTINGHAM.AC.UK
Professor of Medicinal Chemistry and Drug Discovery

Peter M. Fischer



Abstract

© 2020 The Authors. Biomedical Chromatography published by John Wiley & Sons Ltd A simple, sensitive and cost-effective HPLC-UV bioanalytical method for determination of lopinavir (LPV) in rat and human plasma was developed and validated. The plasma sample preparation procedure includes a combination of protein precipitation using cold acetonitrile and liquid–liquid extraction with n-hexane–ethyl acetate (7:3, v/v). A good chromatographic separation was achieved with a Phenomenex Gemini column (C18, 150 mm × 2.0 mm, 5 μm) at 40°C with gradient elution, at 211 nm. Calibration curves were linear in the range 10–10,000 ng/mL, with a lower limit of quantification of 10 ng/mL using 100 μL of plasma. The accuracy and precision in all validation experiments were within the criteria range set by the guidelines of the Food and Drug Administration. This method was successfully applied to a preliminary pharmacokinetic study in rats following an intravenous bolus administration of LPV. Moreover, the method was subsequently fully validated for human plasma, allowing its use in therapeutic drug monitoring (TDM). In conclusion, this novel, simple and cost-efficient bioanalytical method for determination of LPV is useful for pharmacokinetic and drug delivery studies in rats, as well as TDM in human patients.

Journal Article Type Article
Acceptance Date Jun 24, 2020
Online Publication Date Jul 10, 2020
Publication Date 2020-11
Deposit Date Jul 8, 2020
Publicly Available Date Jul 11, 2021
Journal Biomedical Chromatography
Print ISSN 0269-3879
Electronic ISSN 1099-0801
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 34
Issue 11
Article Number e4934
DOI https://doi.org/10.1002/bmc.4934
Keywords Analytical Chemistry; Clinical Biochemistry; Biochemistry; Molecular Biology; Pharmacology; Drug Discovery; General Medicine
Public URL https://nottingham-repository.worktribe.com/output/4747375
Publisher URL https://onlinelibrary.wiley.com/doi/abs/10.1002/bmc.4934

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