Positioning the Root Elongation Zone Is Saltatory and Receives Input from the Shoot

Baskin, Tobias I.; Preston, Simon; Zelinsky, Ellen; Yang, Xiaoli; Elmali, Melissa; Bellos, Dimitrios; Wells, Darren M.; Bennett, Malcolm J.

Abstract

In the root, meristem and elongation zone lengths remain stable, despite growth and division of cells. To gain insight into zone stability, we imaged individual Arabidopsis thaliana roots through a horizontal microscope, and used image analysis to obtain velocity profiles. For a root, velocity profiles obtained every 5 min over 3 h coincided closely, implying that zonation is regulated tightly. However, the position of the elongation zone saltated, by on average 17 μm every 5 min. Saltation was apparently driven by material elements growing faster and then slower, while moving through the growth zone. When the shoot was excised, after about 90 minutes, growth zone dynamics resembled those of intact roots, except that the position of the elongation zone moved, on average, rootward, by several hundred microns in 24 h. We hypothesize that mechanisms determining elongation zone position receive input from the shoot.

Citation

Baskin, T. I., Preston, S., Zelinsky, E., Yang, X., Elmali, M., Bellos, D., Wells, D. M., & Bennett, M. J. (2020). Positioning the Root Elongation Zone Is Saltatory and Receives Input from the Shoot. iScience, 23(7), Article 101309. https://doi.org/10.1016/j.isci.2020.101309

• Journal Article Type: Article
• Acceptance Date: Jun 18, 2020
• Online Publication Date: Jun 24, 2020
• Publication Date: Jul 24, 2020
• Deposit Date: Jun 29, 2020
• Publicly Available Date: Jun 30, 2020
• Journal: iScience
• Electronic ISSN: 2589-0042
• Publisher: Cell Press
• Peer Reviewed: Peer Reviewed
• Volume: 23
• Issue: 7
• Article Number: 101309
• DOI: https://doi.org/10.1016/j.isci.2020.101309
• Public URL: https://nottingham-repository.worktribe.com/output/4736840
• Publisher URL: https://www.cell.com/iscience/fulltext/S2589-0042(20)30496-X