Hypoglycin A absorption in sheep without concurrent clinical or biochemical evidence of disease

González‐Medina, Sonia; Bevin, William; Alzola‐Domingo, Rafael; Chang, Yu‐Mei; Piercy, Richard J.

Abstract

Background

Hypoglycin A (HGA) intoxication after ingestion of Acer spp. tree material has never been confirmed in domesticated ruminants despite their similar grazing habitats.

Objectives

To investigate whether sheep have low HGA bioavailability caused by rumen HGA breakdown.

Animals

Stomach and rumen fluid samples from 5 adult horses and 5 adult sheep respectively. Residual serum samples from 30 ewes and lambs.

Methods

Experimental and retrospective cohort study. Hypoglycin A concentration was quantified in horse gastric and sheep ruminal samples after in vitro incubation with Acer pseudoplatanus seeds. Serum samples from grazing sheep (n = 20) and nursing lambs (n = 10) obtained before and after their release onto pastures with and without Sycamore seedlings were analyzed for HGA and methylenecyclopropyl-acetic acid carnitine, and serum biochemistry.

Results

Neither ovine rumen nor equine gastric fluid affected HGA content in samples incubated for up to 2 hours. Despite HGA's detection in serum from sheep (n = 13/15; median, 23.71 ng/mL; range, 5.62-126.4 ng/mL) grazing contaminated pastures and in their nursing lambs (n = 2/5; median, 12.5 ng/mL; range, 8.82-15.67 ng/mL), there was no apparent clinical or subclinical disease.

Conclusions and Clinical Importance

Any reduced sensitivity to HGA intoxication in sheep seems unrelated to ruminal degradation. Serum HGA concentrations in sheep were similar to those of subclinically affected atypical myopathy horses. Any reduced sensitivity of sheep to HGA might be related to greater metabolic resistance rather than selective grazing habits or lower bioavailability. Hypoglycin A was found in nursing lambs, suggesting that HGA is excreted in milk.

Citation

González‐Medina, S., Bevin, W., Alzola‐Domingo, R., Chang, Y., & Piercy, R. J. (2021). Hypoglycin A absorption in sheep without concurrent clinical or biochemical evidence of disease. Journal of Veterinary Internal Medicine, 35(2), 1170-1176. https://doi.org/10.1111/jvim.16077

• Journal Article Type: Article
• Acceptance Date: Feb 4, 2021
• Online Publication Date: Mar 6, 2021
• Publication Date: Mar 1, 2021
• Deposit Date: Mar 28, 2025
• Publicly Available Date: Mar 28, 2025
• Journal: Journal of Veterinary Internal Medicine
• Print ISSN: 0891-6640
• Electronic ISSN: 1939-1676
• Publisher: Wiley Open Access
• Peer Reviewed: Peer Reviewed
• Volume: 35
• Issue: 2
• Pages: 1170-1176
• DOI: https://doi.org/10.1111/jvim.16077
• Public URL: https://nottingham-repository.worktribe.com/output/47005749
• Publisher URL: https://onlinelibrary.wiley.com/doi/10.1111/jvim.16077
• Additional Information: Received: 2020-07-17; Accepted: 2021-02-04; Published: 2021-03-06