Isabel C. Nimmo
Genetic transformation of the dinoflagellate chloroplast
Nimmo, Isabel C.; Barbrook, Adrian C.; Lassadi, Imen; Chen, Jit Ern; Geisler, Katrin; Smith, Alison G.; Aranda, Manuel; Purton, Saul; Waller, Ross F.; Nisbet, R Ellen R.; Howe, Christopher J.
Authors
Adrian C. Barbrook
Imen Lassadi
Jit Ern Chen
Katrin Geisler
Alison G. Smith
Manuel Aranda
Saul Purton
Ross F. Waller
Dr ELLEN NISBET ELLEN.NISBET@NOTTINGHAM.AC.UK
ASSOCIATE PROFESSOR
Christopher J. Howe
Abstract
Coral reefs are some of the most important and ecologically diverse marine environments. At the base of the reef ecosystem are dinoflagellate algae, which live symbiotically within coral cells. Efforts to understand the relationship between alga and coral have been greatly hampered by the lack of an appropriate dinoflagellate genetic transformation technology. By making use of the plasmid-like fragmented chloroplast genome, we have introduced novel genetic material into the dinoflagellate chloroplast genome. We have shown that the introduced genes are expressed and confer the expected phenotypes. Genetically modified cultures have been grown for 1 year with subculturing, maintaining the introduced genes and phenotypes. This indicates that cells continue to divide after transformation and that the transformation is stable. This is the first report of stable chloroplast transformation in dinoflagellate algae.
Citation
Nimmo, I. C., Barbrook, A. C., Lassadi, I., Chen, J. E., Geisler, K., Smith, A. G., Aranda, M., Purton, S., Waller, R. F., Nisbet, R. E. R., & Howe, C. J. (2019). Genetic transformation of the dinoflagellate chloroplast. eLife, 8, Article e45292. https://doi.org/10.7554/elife.45292
Journal Article Type | Article |
---|---|
Acceptance Date | Jul 6, 2019 |
Online Publication Date | Jul 18, 2019 |
Publication Date | Jul 18, 2019 |
Deposit Date | Jun 12, 2020 |
Publicly Available Date | Jun 18, 2020 |
Journal | eLife |
Electronic ISSN | 2050-084X |
Publisher | eLife Sciences Publications |
Peer Reviewed | Peer Reviewed |
Volume | 8 |
Article Number | e45292 |
DOI | https://doi.org/10.7554/elife.45292 |
Keywords | General Biochemistry, Genetics and Molecular Biology; General Immunology and Microbiology; General Neuroscience; General Medicine |
Public URL | https://nottingham-repository.worktribe.com/output/4633381 |
Publisher URL | https://elifesciences.org/articles/45292 |
Files
Genetic transformation
(1.1 Mb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
You might also like
Multiplexing projects: supervising undergraduate research projects with larger cohorts
(2024)
Journal Article
Evolution: The plasticity of plastids
(2023)
Journal Article
Evolution: The great photosynthesis heist
(2023)
Journal Article
25 years of Protist: A thank you to Editor-in-Chief Michael Melkonian
(2023)
Journal Article
Establishment of a novel set of vectors for transformation of the dinoflagellate Amphidinium carterae
(2022)
Preprint / Working Paper
Downloadable Citations
About Repository@Nottingham
Administrator e-mail: discovery-access-systems@nottingham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search