Skip to main content

Research Repository

Advanced Search

Genetic transformation of the dinoflagellate chloroplast

Nimmo, Isabel C.; Barbrook, Adrian C.; Lassadi, Imen; Chen, Jit Ern; Geisler, Katrin; Smith, Alison G.; Aranda, Manuel; Purton, Saul; Waller, Ross F.; Nisbet, R Ellen R.; Howe, Christopher J.

Genetic transformation of the dinoflagellate chloroplast Thumbnail


Authors

Isabel C. Nimmo

Adrian C. Barbrook

Imen Lassadi

Jit Ern Chen

Katrin Geisler

Alison G. Smith

Manuel Aranda

Saul Purton

Ross F. Waller

Christopher J. Howe



Abstract

Coral reefs are some of the most important and ecologically diverse marine environments. At the base of the reef ecosystem are dinoflagellate algae, which live symbiotically within coral cells. Efforts to understand the relationship between alga and coral have been greatly hampered by the lack of an appropriate dinoflagellate genetic transformation technology. By making use of the plasmid-like fragmented chloroplast genome, we have introduced novel genetic material into the dinoflagellate chloroplast genome. We have shown that the introduced genes are expressed and confer the expected phenotypes. Genetically modified cultures have been grown for 1 year with subculturing, maintaining the introduced genes and phenotypes. This indicates that cells continue to divide after transformation and that the transformation is stable. This is the first report of stable chloroplast transformation in dinoflagellate algae.

Citation

Nimmo, I. C., Barbrook, A. C., Lassadi, I., Chen, J. E., Geisler, K., Smith, A. G., Aranda, M., Purton, S., Waller, R. F., Nisbet, R. E. R., & Howe, C. J. (2019). Genetic transformation of the dinoflagellate chloroplast. eLife, 8, Article e45292. https://doi.org/10.7554/elife.45292

Journal Article Type Article
Acceptance Date Jul 6, 2019
Online Publication Date Jul 18, 2019
Publication Date Jul 18, 2019
Deposit Date Jun 12, 2020
Publicly Available Date Jun 18, 2020
Journal eLife
Electronic ISSN 2050-084X
Publisher eLife Sciences Publications
Peer Reviewed Peer Reviewed
Volume 8
Article Number e45292
DOI https://doi.org/10.7554/elife.45292
Keywords General Biochemistry, Genetics and Molecular Biology; General Immunology and Microbiology; General Neuroscience; General Medicine
Public URL https://nottingham-repository.worktribe.com/output/4633381
Publisher URL https://elifesciences.org/articles/45292

Files






You might also like



Downloadable Citations