Selective Photoinduced Antibacterial Activity of Amoxicillin-Coated Gold Nanoparticles: From One-Step Synthesis to in Vivo Cytocompatibility

Silvero C., M. Jazm�n; Rocca, Diamela M.; de la Villarmois, Emilce Artur; Fournier, Kelsey; Lanterna, Anabel E.; P�rez, Mariela F.; Becerra, M. Cecilia; Scaiano, Juan C.

Abstract

Photoinduced antibacterial gold nanoparticles were developed as an alternative for the treatment of antibiotic-resistant bacteria. Thanks to the amoxicillin coating, they possess high in vivo stability, selectivity for the bacteria wall, a good renal clearance, and are completely nontoxic for eukaryotic cells at the bactericidal concentrations. A simple one-step synthesis of amoxi@AuNP is described at mild temperatures using the antibiotic as both reducing and stabilizing agent. Time-resolved fluorescence microscopy proved these novel nano-photosensitizers, with improved selectivity, are bactericidal but showing excellent biocompatibility toward eukaryotic cells at the same dose (1.5 μg/mL) when co-cultures are analyzed. Their stability in biological media, hemocompatibility, and photo-antibacterial effect against sensitive and antibiotic-resistant Staphylococcus aureus were evaluated in vitro, whereas toxicity, renal clearance, and biodistribution were studied in vivo in male Wistar rats. The use of these nanoparticles to treat antibiotic-resistant infections is promising given their high stability and cytocompatibility.

• Journal Article Type: Article
• Acceptance Date: Jan 17, 2018
• Online Publication Date: Jan 30, 2018
• Publication Date: Jan 31, 2018
• Deposit Date: Jun 22, 2020
• Publicly Available Date: Jul 21, 2020
• Journal: ACS Omega
• Electronic ISSN: 2470-1343
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 3
• Issue: 1
• Pages: 1220-1230
• DOI: https://doi.org/10.1021/acsomega.7b01779
• Public URL: https://nottingham-repository.worktribe.com/output/4442803
• Publisher URL: https://pubs.acs.org/doi/10.1021/acsomega.7b01779
• Additional Information: Copyright © 2018 American Chemical Society