Selective Photoinduced Antibacterial Activity of Amoxicillin-Coated Gold Nanoparticles: From One-Step Synthesis to in Vivo Cytocompatibility

Silvero C., M. Jazm�n; Rocca, Diamela M.; de la Villarmois, Emilce Artur; Fournier, Kelsey; Lanterna, Anabel E.; P�rez, Mariela F.; Becerra, M. Cecilia; Scaiano, Juan C.

Abstract

Photoinduced antibacterial gold nanoparticles were developed as an alternative for the treatment of antibiotic-resistant bacteria. Thanks to the amoxicillin coating, they possess high in vivo stability, selectivity for the bacteria wall, a good renal clearance, and are completely nontoxic for eukaryotic cells at the bactericidal concentrations. A simple one-step synthesis of amoxi@AuNP is described at mild temperatures using the antibiotic as both reducing and stabilizing agent. Time-resolved fluorescence microscopy proved these novel nano-photosensitizers, with improved selectivity, are bactericidal but showing excellent biocompatibility toward eukaryotic cells at the same dose (1.5 μg/mL) when co-cultures are analyzed. Their stability in biological media, hemocompatibility, and photo-antibacterial effect against sensitive and antibiotic-resistant Staphylococcus aureus were evaluated in vitro, whereas toxicity, renal clearance, and biodistribution were studied in vivo in male Wistar rats. The use of these nanoparticles to treat antibiotic-resistant infections is promising given their high stability and cytocompatibility.

Citation

Silvero C., M. J., Rocca, D. M., de la Villarmois, E. A., Fournier, K., Lanterna, A. E., Pérez, M. F., Becerra, M. C., & Scaiano, J. C. (2018). Selective Photoinduced Antibacterial Activity of Amoxicillin-Coated Gold Nanoparticles: From One-Step Synthesis to in Vivo Cytocompatibility. ACS Omega, 3(1), 1220-1230. https://doi.org/10.1021/acsomega.7b01779

• Journal Article Type: Article
• Acceptance Date: Jan 17, 2018
• Online Publication Date: Jan 30, 2018
• Publication Date: Jan 31, 2018
• Deposit Date: Jun 22, 2020
• Publicly Available Date: Jul 21, 2020
• Journal: ACS Omega
• Electronic ISSN: 2470-1343
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 3
• Issue: 1
• Pages: 1220-1230
• DOI: https://doi.org/10.1021/acsomega.7b01779
• Public URL: https://nottingham-repository.worktribe.com/output/4442803
• Publisher URL: https://pubs.acs.org/doi/10.1021/acsomega.7b01779
• Additional Information: Copyright © 2018 American Chemical Society