Apparent attenuation by opto-acoustic defocus in phonon microscopy

Fuentes-Dom�nguez, Rafael; La Cavera, Salvatore; P�rez-Cota, Fernando; La Cavera III, Salvatore; Naznin, Shakila; Fuentes-Dominguez, Rafael; Smith, Richard J.; Clark, Matt

Abstract

© 2020 The Author(s) Understanding the mechanical properties of biological cells is a challenging problem for the life sciences partly because there are limited methods for mapping elasticity with high resolution. Phonon microscopy is a form of Brillouin light scattering which uses coherent phonons for imaging with elasticity-related contrast, phonon resolution and without labels. It can measure material properties such as sound velocity, acoustic impedance and attenuation. To use it as a contrast mechanism in microscopy, high numerical aperture (NA) lenses are key to high resolution. However, increasing NA induces apparent attenuation, a premature decay of the detected signal. To reduce signal decay and quantify the sound attenuation coefficient in cells, it is necessary to understand the mechanisms that affect signal decay. Here we define opto-acoustic defocus as a signal decay mechanism and propose methods to achieve quantitative sound attenuation measurements, and to optimise in-depth imaging at high resolution which is crucial for cell imaging.

Citation

Fuentes-Domínguez, R., La Cavera, S., Pérez-Cota, F., La Cavera III, S., Naznin, S., Fuentes-Dominguez, R., Smith, R. J., & Clark, M. (2020). Apparent attenuation by opto-acoustic defocus in phonon microscopy. Photoacoustics, 19, Article 100180. https://doi.org/10.1016/j.pacs.2020.100180

• Journal Article Type: Article
• Acceptance Date: Mar 30, 2020
• Online Publication Date: May 17, 2020
• Publication Date: Sep 1, 2020
• Deposit Date: Apr 27, 2020
• Publicly Available Date: May 17, 2020
• Journal: Photoacoustics
• Electronic ISSN: 2213-5979
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 19
• Article Number: 100180
• DOI: https://doi.org/10.1016/j.pacs.2020.100180
• Keywords: Atomic and Molecular Physics, and Optics; Radiology Nuclear Medicine and imaging
• Public URL: https://nottingham-repository.worktribe.com/output/4348964
• Additional Information: This article is maintained by: Elsevier; Article Title: Apparent attenuation by opto-acoustic defocus in phonon microscopy; Journal Title: Photoacoustics; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.pacs.2020.100180; Content Type: article; Copyright: © 2020 The Author(s). Published by Elsevier GmbH.