Exploiting the genome of Thinopyrum elongatum to expand the gene pool of hexaploid wheat

Baker, Lauren; Grewal, Surbhi; Yang, Cai yun; Hubbart-Edwards, Stella; Scholefield, Duncan; Ashling, Stephen; Burridge, Amanda J.; Przewieslik-Allen, Alexandra M.; Wilkinson, Paul A.; King, Ian P.; King, Julie

Abstract

© 2020, The Author(s). Key message: One hundred and thirty four introgressions from Thinopyrum elongatum have been transferred into a wheat background and were characterised using 263 SNP markers. Abstract: Species within the genus Thinopyrum have been shown to carry genetic variation for a very wide range of traits including biotic and abiotic stresses and quality. Research has shown that one of the species within this genus, Th. elongatum, has a close relationship with the genomes of wheat making it a highly suitable candidate to expand the gene pool of wheat. Homoeologous recombination, in the absence of the Ph1 gene, has been exploited to transfer an estimated 134 introgressions from Th. elongatum into a hexaploid wheat background. The introgressions were detected and characterised using 263 single nucleotide polymorphism markers from a 35K Axiom® Wheat-Relative Genotyping Array, spread across seven linkage groups and validated using genomic in situ hybridisation. The genetic map had a total length of 187.8cM and the average chromosome length was 26.8cM. Comparative analyses of the genetic map of Th. elongatum and the physical map of hexaploid wheat confirmed previous work that indicated good synteny at the macro-level, although Th. elongatum does not contain the 4A/5A/7B translocation found in wheat.

Citation

Baker, L., Grewal, S., Yang, C. Y., Hubbart-Edwards, S., Scholefield, D., Ashling, S., …King, J. (2020). Exploiting the genome of Thinopyrum elongatum to expand the gene pool of hexaploid wheat. TAG Theoretical and Applied Genetics, 133, 2213–2226. https://doi.org/10.1007/s00122-020-03591-3

• Journal Article Type: Article
• Acceptance Date: Mar 31, 2020
• Online Publication Date: Apr 20, 2020
• Publication Date: Jul 1, 2020
• Deposit Date: Apr 20, 2020
• Publicly Available Date: Apr 21, 2020
• Journal: Theoretical and Applied Genetics
• Print ISSN: 0040-5752
• Electronic ISSN: 1432-2242
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 133
• Pages: 2213–2226
• DOI: https://doi.org/10.1007/s00122-020-03591-3
• Keywords: Biotechnology; Agronomy and Crop Science; Genetics; General Medicine
• Public URL: https://nottingham-repository.worktribe.com/output/4316211
• Publisher URL: https://link.springer.com/article/10.1007%2Fs00122-020-03591-3
• Additional Information: Received: 29 October 2019; Accepted: 31 March 2020; First Online: 20 April 2020; : ; : On behalf of all authors, the corresponding author states that there is no conflict of interest.