Genetic tool development in marine protists: emerging model organisms for experimental cell biology

Faktorov?, Drahom?ra; Nisbet, R. Ellen R.; Fern?ndez Robledo, Jos? A.; Casacuberta, Elena; Sudek, Lisa; Allen, Andrew E.; Ares, Manuel; Arest?, Cristina; Balestreri, Cecilia; Barbrook, Adrian C.; Beardslee, Patrick; Bender, Sara; Booth, David S.; Bouget, Fran?ois-Yves; Bowler, Chris; Breglia, Susana A.; Brownlee, Colin; Burger, Gertraud; Cerutti, Heriberto; Cesaroni, Rachele; Chiurillo, Miguel A.; Clemente, Thomas; Coles, Duncan B.; Collier, Jackie L.; Cooney, Elizabeth C.; Coyne, Kathryn; Docampo, Roberto; Dupont, Christopher L.; Edgcomb, Virginia; Einarsson, Elin; Elustondo, P?a A.; Federici, Fernan; Freire-Beneitez, Veronica; Freyria, Nastasia J.; Fukuda, Kodai; Garc?a, Paulo A.; Girguis, Peter R.; Gomaa, Fatma; Gornik, Sebastian G.; Guo, Jian; Hampl, Vladim?r; Hanawa, Yutaka; Haro-Contreras, Esteban R.; Hehenberger, Elisabeth; Highfield, Andrea; Hirakawa, Yoshihisa; Hopes, Amanda; Howe, Christopher J.; Hu, Ian; Iba?ez, Jorge; Irwin, Nicholas A. T.; Ishii, Yuu; Janowicz, Natalia Ewa...

Abstract

Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.

Citation

Faktorová, D., Nisbet, R. E. R., Fernández Robledo, J. A., Casacuberta, E., Sudek, L., Allen, A. E., Ares, M., Aresté, C., Balestreri, C., Barbrook, A. C., Beardslee, P., Bender, S., Booth, D. S., Bouget, F.-Y., Bowler, C., Breglia, S. A., Brownlee, C., Burger, G., Cerutti, H., Cesaroni, R., …Lukeš, J. (2020). Genetic tool development in marine protists: emerging model organisms for experimental cell biology. Nature Methods, 17, 481–494. https://doi.org/10.1038/s41592-020-0796-x

• Journal Article Type: Article
• Acceptance Date: Apr 2, 2020
• Online Publication Date: Apr 6, 2020
• Publication Date: 2020-05
• Deposit Date: Apr 16, 2020
• Publicly Available Date: Apr 16, 2020
• Journal: Nature Methods
• Print ISSN: 1548-7091
• Electronic ISSN: 1548-7105
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 17
• Pages: 481–494
• DOI: https://doi.org/10.1038/s41592-020-0796-x
• Keywords: Biotechnology; Cell Biology; Biochemistry; Molecular Biology
• Public URL: https://nottingham-repository.worktribe.com/output/4298100
• Publisher URL: https://www.nature.com/articles/s41592-020-0796-x
• Additional Information: Received: 26 June 2019; Accepted: 2 March 2020; First Online: 6 April 2020; Change Date: 15 April 2020; Change Type: Correction; Change Details: An amendment to this paper has been published and can be accessed via a link at the top of the paper.; : The authors declare no competing interests.