Genetic tool development in marine protists: emerging model organisms for experimental cell biology

Faktorov�, Drahom�ra; Nisbet, R. Ellen R.; Fern�ndez Robledo, Jos� A.; Casacuberta, Elena; Sudek, Lisa; Allen, Andrew E.; Ares, Manuel; Arest�, Cristina; Balestreri, Cecilia; Barbrook, Adrian C.; Beardslee, Patrick; Bender, Sara; Booth, David S.; Bouget, Fran�ois-Yves; Bowler, Chris; Breglia, Susana A.; Brownlee, Colin; Burger, Gertraud; Cerutti, Heriberto; Cesaroni, Rachele; Chiurillo, Miguel A.; Clemente, Thomas; Coles, Duncan B.; Collier, Jackie L.; Cooney, Elizabeth C.; Coyne, Kathryn; Docampo, Roberto; Dupont, Christopher L.; Edgcomb, Virginia; Einarsson, Elin; Elustondo, P�a A.; Federici, Fernan; Freire-Beneitez, Veronica; Freyria, Nastasia J.; Fukuda, Kodai; Garc�a, Paulo A.; Girguis, Peter R.; Gomaa, Fatma; Gornik, Sebastian G.; Guo, Jian; Hampl, Vladim�r; Hanawa, Yutaka; Haro-Contreras, Esteban R.; Hehenberger, Elisabeth; Highfield, Andrea; Hirakawa, Yoshihisa; Hopes, Amanda; Howe, Christopher J.; Hu, Ian; Iba�ez, Jorge; Irwin, Nicholas A. T.; Ishii, Yuu; Janowicz, Natalia Ewa; Jones, Adam C.; Kachale, Ambar; Fujimura-Kamada, Konomi; Kaur, Binnypreet; Kaye, Jonathan Z.; Kazana, Eleanna; Keeling, Patrick J.; King, Nicole; Klobutcher, Lawrence A.; Lander, Noelia; Lassadi, Imen; Li, Zhuhong; Lin, Senjie; Lozano, Jean-Claude; Luan, Fulei; Maruyama, Shinichiro; Matute, Tamara; Miceli, Cristina; Minagawa, Jun; Moosburner, Mark; Najle, Sebasti�n R.; Nanjappa, Deepak; Nimmo, Isabel C.; Noble, Luke; Nov�k Vanclov�, Anna M. G.; Nowacki, Mariusz; Nu�ez, Isaac; Pain, Arnab; Piersanti, Angela; Pucciarelli, Sandra; Pyrih, Jan; Rest, Joshua S.; Rius, Mariana; Robertson, Deborah; Ruaud, Albane; Ruiz-Trillo, I�aki; Sigg, Monika A.; Silver, Pamela A.; Slamovits, Claudio H.; Jason Smith, G.; Sprecher, Brittany N.; Stern, Rowena; Swart, Estienne C.; Tsaousis, Anastasios D.; Tsypin, Lev; Turkewitz, Aaron; Turn�ek, Jernej; Valach, Matus; Verg�, Val�rie; von Dassow, Peter; von der Haar, Tobias; Waller, Ross F.; Wang, Lu; Wen, Xiaoxue; Wheeler, Glen; Woods, April; Zhang, Huan; Mock, Thomas; Worden, Alexandra Z.; Luke�, Julius

doi:10.1038/s41592-020-0796-x

Genetic tool development in marine protists: emerging model organisms for experimental cell biology

Faktorov?, Drahom?ra; Nisbet, R. Ellen R.; Fern?ndez Robledo, Jos? A.; Casacuberta, Elena; Sudek, Lisa; Allen, Andrew E.; Ares, Manuel; Arest?, Cristina; Balestreri, Cecilia; Barbrook, Adrian C.; Beardslee, Patrick; Bender, Sara; Booth, David S.; Bouget, Fran?ois-Yves; Bowler, Chris; Breglia, Susana A.; Brownlee, Colin; Burger, Gertraud; Cerutti, Heriberto; Cesaroni, Rachele; Chiurillo, Miguel A.; Clemente, Thomas; Coles, Duncan B.; Collier, Jackie L.; Cooney, Elizabeth C.; Coyne, Kathryn; Docampo, Roberto; Dupont, Christopher L.; Edgcomb, Virginia; Einarsson, Elin; Elustondo, P?a A.; Federici, Fernan; Freire-Beneitez, Veronica; Freyria, Nastasia J.; Fukuda, Kodai; Garc?a, Paulo A.; Girguis, Peter R.; Gomaa, Fatma; Gornik, Sebastian G.; Guo, Jian; Hampl, Vladim?r; Hanawa, Yutaka; Haro-Contreras, Esteban R.; Hehenberger, Elisabeth; Highfield, Andrea; Hirakawa, Yoshihisa; Hopes, Amanda; Howe, Christopher J.; Hu, Ian; Iba?ez, Jorge; Irwin, Nicholas A. T.; Ishii, Yuu; Janowicz, Natalia Ewa...

Authors

Drahom�ra Faktorov�

ELLEN NISBET ELLEN.NISBET@NOTTINGHAM.AC.UK
Associate Professor

Jos� A. Fern�ndez Robledo

Elena Casacuberta

Lisa Sudek

Andrew E. Allen

Manuel Ares

Cristina Arest�

Cecilia Balestreri

Adrian C. Barbrook

Patrick Beardslee

Sara Bender

David S. Booth

Fran�ois-Yves Bouget

Chris Bowler

Susana A. Breglia

Colin Brownlee

Gertraud Burger

Heriberto Cerutti

Rachele Cesaroni

Miguel A. Chiurillo

Thomas Clemente

Duncan B. Coles

Jackie L. Collier

Elizabeth C. Cooney

Kathryn Coyne

Roberto Docampo

Christopher L. Dupont

Virginia Edgcomb

Elin Einarsson

P�a A. Elustondo

Fernan Federici

Veronica Freire-Beneitez

Nastasia J. Freyria

Kodai Fukuda

Paulo A. Garc�a

Peter R. Girguis

Fatma Gomaa

Sebastian G. Gornik

Jian Guo

Vladim�r Hampl

Yutaka Hanawa

Esteban R. Haro-Contreras

Elisabeth Hehenberger

Andrea Highfield

Yoshihisa Hirakawa

Amanda Hopes

Christopher J. Howe

Ian Hu

Jorge Iba�ez

Nicholas A. T. Irwin

Yuu Ishii

Natalia Ewa Janowicz

Adam C. Jones

Ambar Kachale

Konomi Fujimura-Kamada

Binnypreet Kaur

Jonathan Z. Kaye

Eleanna Kazana

Patrick J. Keeling

Nicole King

Lawrence A. Klobutcher

Noelia Lander

Imen Lassadi

Zhuhong Li

Senjie Lin

Jean-Claude Lozano

Fulei Luan

Shinichiro Maruyama

Tamara Matute

Cristina Miceli

Jun Minagawa

Mark Moosburner

Sebasti�n R. Najle

Deepak Nanjappa

Isabel C. Nimmo

Luke Noble

Anna M. G. Nov�k Vanclov�

Mariusz Nowacki

Isaac Nu�ez

Arnab Pain

Angela Piersanti

Sandra Pucciarelli

Jan Pyrih

Joshua S. Rest

Mariana Rius

Deborah Robertson

Albane Ruaud

I�aki Ruiz-Trillo

Monika A. Sigg

Pamela A. Silver

Claudio H. Slamovits

G. Jason Smith

Brittany N. Sprecher

Rowena Stern

Estienne C. Swart

Anastasios D. Tsaousis

Lev Tsypin

Aaron Turkewitz

Jernej Turn�ek

Matus Valach

Val�rie Verg�

Peter von Dassow

Tobias von der Haar

Ross F. Waller

Lu Wang

Xiaoxue Wen

Glen Wheeler

April Woods

Huan Zhang

Thomas Mock

Alexandra Z. Worden

Julius Luke�

Abstract

Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.

Citation

Faktorová, D., Nisbet, R. E. R., Fernández Robledo, J. A., Casacuberta, E., Sudek, L., Allen, A. E., …Lukeš, J. (2020). Genetic tool development in marine protists: emerging model organisms for experimental cell biology. Nature Methods, 17, 481–494. https://doi.org/10.1038/s41592-020-0796-x

Journal Article Type	Article
Acceptance Date	Apr 2, 2020
Online Publication Date	Apr 6, 2020
Publication Date	2020-05
Deposit Date	Apr 16, 2020
Publicly Available Date	Apr 16, 2020
Journal	Nature Methods
Print ISSN	1548-7091
Electronic ISSN	1548-7105
Publisher	Nature Publishing Group
Peer Reviewed	Peer Reviewed
Volume	17
Pages	481–494
DOI	https://doi.org/10.1038/s41592-020-0796-x
Keywords	Biotechnology; Cell Biology; Biochemistry; Molecular Biology
Public URL	https://nottingham-repository.worktribe.com/output/4298100
Publisher URL	https://www.nature.com/articles/s41592-020-0796-x
Additional Information	Received: 26 June 2019; Accepted: 2 March 2020; First Online: 6 April 2020; Change Date: 15 April 2020; Change Type: Correction; Change Details: An amendment to this paper has been published and can be accessed via a link at the top of the paper.; : The authors declare no competing interests.