The effects of exogenous xylanase supplementation on the in vivo generation of xylooligosaccharides and monosaccharides in broilers fed a wheat-based diet

Dale, Tom; Hannay, Isabella; Bedford, Michael R; Tucker, Gregory A; Brameld, John; Parr, Tim

Abstract

1. This study aimed to quantify xylanase-induced changes in soluble monosaccharides, xylooligosaccharides (XOS) and volatile fatty acid (VFA) contents of the different sections of the GIT and whether these relate to altered bird performance.
2. An in vitro digestion of the wheat-based diet was carried out with the xylanase (Econase XT at 16,000BXU/kg diet) to compare the in vitro and in vivo generation of these XOS and monosaccharides. For the in vivo study, 80 male Ross 508 broiler chicks were split into two groups fed a wheat-based diet with or without Econase XT (16,000BXU/kg diet) for 21 days.
3. There were no effects of Econase XT inclusion on growth performance characteristics, likely a result of the high-quality wheat diet and corresponding high performance of the control group (FCR average of 1.45 in controls), but also the relatively young age (from 4 to 26 days of age).
4. Econase XT supplementation increased the xylotetraose (X4) content in the colon (p=0.046, enzyme x GIT section interaction) and the xylose contents in the colon and caeca (p[less than]0.001, enzyme x GIT section interaction).
5. The trend for increased acetate proportion in the caeca of Econase XT treated birds (p=0.062) suggests that the XOS generated were subsequently fermented in the caeca, potentially impacting upon the types of microbiota present.
6. The present study suggests that wheat arabinoxylan degradation is enhanced by xylanase supplementation, which may increase the production of beneficial VFA in the caeca, and thereby potentially modulate the caecal microbiome, but without affecting bird performance (at this early stage).

Citation

Dale, T., Hannay, I., Bedford, M. R., Tucker, G. A., Brameld, J., & Parr, T. (2020). The effects of exogenous xylanase supplementation on the in vivo generation of xylooligosaccharides and monosaccharides in broilers fed a wheat-based diet. British Poultry Science, 61(4), 471-481. https://doi.org/10.1080/00071668.2020.1751805

• Journal Article Type: Article
• Acceptance Date: Feb 21, 2020
• Online Publication Date: Jul 20, 2020
• Publication Date: 2020
• Deposit Date: Apr 14, 2020
• Publicly Available Date: Jul 21, 2021
• Journal: British Poultry Science
• Print ISSN: 0007-1668
• Electronic ISSN: 1466-1799
• Publisher: Taylor and Francis
• Peer Reviewed: Peer Reviewed
• Volume: 61
• Issue: 4
• Pages: 471-481
• DOI: https://doi.org/10.1080/00071668.2020.1751805
• Keywords: XOS; enzymes; chicken; microbiome; non-ruminant
• Public URL: https://nottingham-repository.worktribe.com/output/4292679
• Publisher URL: https://www.tandfonline.com/doi/full/10.1080/00071668.2020.1751805
• Additional Information: This is an Accepted Manuscript of an article published by Taylor & Francis in British Poultry Science on [date of publication], available online: http://www.tandfonline.com/10.1080/00071668.2020.1751805