Tissue-specific expression of p73 C-terminal isoforms in mice

Grespi, Francesca; Amelio, Ivano; Tucci, Paola; Annicchiarico-Petruzzelli, Margherita; Melino, Gerry

Abstract

p73 is a p53 family transcription factor. Due to the presence in the 5′ flanking region of two promoters, there are two N-terminal variants, TAp73, which retains a fully active transactivation domain (TA), and ΔNp73, in which the N terminus is truncated. In addition, extensive 3′ splicing gives rise to at least seven distinctive isoforms; TAp73-selective knockout highlights its role as a regulator of cell death, senescence and tumor suppressor. ΔNp73-selective knockout, on the other hand, highlights anti-apoptotic function of ΔNp73 and its involvement in DNA damage response. In this work, we investigated the expression pattern of murine p73 C-terminal isoforms. By using a RT-PCR approach, we were able to detect mRNAs of all the C-terminal isoforms described in humans. We characterized their in vivo expression profile in mouse organs and in different mouse developmental stages. Finally, we investigated p73 C-terminal expression profile following DNA damage, ex vivo after primary cultures treatment and in vivo after systemic administration of cytotoxic compounds. Overall, our study first elucidates spatio-temporal expression of mouse p73 isoforms and provides novel insights on their expression-switch under triggered conditions.

Citation

Grespi, F., Amelio, I., Tucci, P., Annicchiarico-Petruzzelli, M., & Melino, G. (2012). Tissue-specific expression of p73 C-terminal isoforms in mice. Cell Cycle, 11(23), 4474-4483. https://doi.org/10.4161/cc.22787

• Journal Article Type: Article
• Online Publication Date: Oct 28, 2014
• Publication Date: 2012-12
• Deposit Date: Apr 1, 2020
• Journal: Cell Cycle
• Print ISSN: 1538-4101
• Electronic ISSN: 1551-4005
• Publisher: Taylor and Francis
• Peer Reviewed: Peer Reviewed
• Volume: 11
• Issue: 23
• Pages: 4474-4483
• DOI: https://doi.org/10.4161/cc.22787
• Keywords: C-terminal isoforms, SAM domain, cancer, development, p73
• Public URL: https://nottingham-repository.worktribe.com/output/4237372