Construction of synthetic regulatory networks in yeast

Blount, Benjamin A.; Weenink, Tim; Ellis, Tom

Abstract

Yeast species such as Saccharomyces cerevisiae have been exploited by humans for millennia and so it is therefore unsurprising that they are attractive cells to re-engineer for industrial use. Despite many beneficial traits yeast has for synthetic biology, it currently lags behind Escherichia coli in the number of synthetic networks that have been described. While the eukaryotic nature of yeast means that its regulation is not as simple to predict as it is for E. coli, once initial considerations have been made yeast is pleasingly tractable. In this review we provide a loose guide for constructing and implementing synthetic regulatory networks in S. cerevisiae using examples from previous research to highlight available resources, specific considerations and potential future advances. © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Citation

Blount, B. A., Weenink, T., & Ellis, T. (2012). Construction of synthetic regulatory networks in yeast. FEBS Letters, 586(15), 2112-2121. https://doi.org/10.1016/j.febslet.2012.01.053

• Journal Article Type: Review
• Acceptance Date: Jan 26, 2012
• Online Publication Date: Feb 2, 2012
• Publication Date: Jul 16, 2012
• Deposit Date: Aug 3, 2022
• Publicly Available Date: Aug 5, 2022
• Journal: FEBS Letters
• Print ISSN: 0014-5793
• Electronic ISSN: 1873-3468
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 586
• Issue: 15
• Pages: 2112-2121
• DOI: https://doi.org/10.1016/j.febslet.2012.01.053
• Public URL: https://nottingham-repository.worktribe.com/output/4183561
• Publisher URL: https://febs.onlinelibrary.wiley.com/doi/10.1016/j.febslet.2012.01.053