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Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functions

Prigge, Michael J; Platre, Matthieu; Kadakia, Nikita; Zhang, Yi; Greenham, Kathleen; Szutu, Whitnie; Pandey, Bipin Kumar; Bhosale, Rahul Arvind; Bennett, Malcolm J.; Busch, Wolfgang; Estelle, Mark


Michael J Prigge

Matthieu Platre

Nikita Kadakia

Yi Zhang

Kathleen Greenham

Whitnie Szutu

Beacon Nottingham Research Fellow

Wolfgang Busch

Mark Estelle


© 2020, Prigge et al. The TIR1/AFB auxin co-receptors mediate diverse responses to the plant hormone auxin. The Arabidopsis genome encodes six TIR1/AFB proteins representing three of the four clades that were established prior to angiosperm radiation. To determine the role of these proteins in plant development we performed an extensive genetic analysis involving the generation and characterization of all possible multiply-mutant lines. We find that loss of all six TIR1/AFB proteins results in early embryo defects and eventually seed abortion, and yet a single wild-type allele of TIR1 or AFB2 is sufficient to support growth throughout development. Our analysis reveals extensive functional overlap between even the most distantly related TIR1/AFB genes except for AFB1. Surprisingly, AFB1 has a specialized function in rapid auxin-dependent inhibition of root growth and early phase of root gravitropism. This activity may be related to a difference in subcellular localization compared to the other members of the family.


Prigge, M. J., Prigge, M. J., Platre, M., Kadakia, N., Zhang, Y., Greenham, K., …Estelle, M. (2020). Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functions. eLife, 9,

Journal Article Type Article
Acceptance Date Feb 4, 2020
Online Publication Date Feb 18, 2020
Publication Date Feb 18, 2020
Deposit Date Feb 21, 2020
Publicly Available Date Mar 19, 2020
Journal eLife
Electronic ISSN 2050-084X
Publisher eLife Sciences Publications
Peer Reviewed Peer Reviewed
Volume 9
Article Number e54740
Keywords General Biochemistry, Genetics and Molecular Biology; General Immunology and Microbiology; General Neuroscience; General Medicine
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