The impact of a high-fat diet in mice is dependent on duration and age, and differs between muscles

Messa, G. A. M.; Piasecki, Mathew; Hurst, Josh; Hill, Cameron; Tallis, Jason; Degens, Hans

Abstract

© 2020. Published by The Company of Biologists Ltd. Prolonged high-fat diets (HFDs) can cause intramyocellular lipid (IMCL) accumulation that may negatively affect muscle function. We investigated the duration of a HFD required to instigate these changes, and whether the effects are muscle specific and aggravated in older age. Muscle morphology was determined in the soleus, extensor digitorum longus (EDL) and diaphragm muscles of female CD-1 mice from 5 groups: Young fed a HFD for 8 weeks (YS-HFD, n=16), young fed a HFD for 16 weeks (YL-HFD, n=28) and young control (Y-Con, n=28). The young animals were 20 weeks old at the end of the experiment. Old (70weeks) femaleCD-1 mice received either a normal diet (O-Con, n=30) or a HFD for 9 weeks (OS-HFD, n=30). Body mass, body mass index and intramyocellular lipid (IMCL) content increased in OS-HFD (P≤0.003). In the young mice, this increase was seen in YL-HFD and not YS-HFD (P≤0.006). The soleus and diaphragm fibre cross-sectional area (FCSA) in YL-HFD was larger than that in Y-Con (P≤0.004)whileOS-HFDhad a larger soleus FCSA compared with that of O-Con after only 9 weeks on a HFD(P

Citation

Messa, G. A. M., Piasecki, M., Hurst, J., Hill, C., Tallis, J., & Degens, H. (2020). The impact of a high-fat diet in mice is dependent on duration and age, and differs between muscles. Journal of Experimental Biology, 223(6), https://doi.org/10.1242/jeb.217117

• Journal Article Type: Article
• Acceptance Date: Jan 20, 2020
• Online Publication Date: Jan 27, 2020
• Publication Date: Mar 1, 2020
• Deposit Date: Jan 29, 2020
• Publicly Available Date: Mar 29, 2020
• Journal: Journal of Experimental Biology
• Print ISSN: 0022-0949
• Electronic ISSN: 1477-9145
• Publisher: Company of Biologists
• Peer Reviewed: Peer Reviewed
• Volume: 223
• Issue: 6
• Article Number: jeb.217117
• DOI: https://doi.org/10.1242/jeb.217117
• Keywords: Insect Science; Animal Science and Zoology; Aquatic Science; Physiology; Ecology, Evolution, Behavior and Systematics; Molecular Biology
• Public URL: https://nottingham-repository.worktribe.com/output/3828778
• Publisher URL: https://jeb.biologists.org/content/early/2020/01/25/jeb.217117