Dual predation by bacteriophage and bdellovibrio bacteriovorus can eradicate escherichia coli prey in situations where single predation cannot

Hobley, Laura; Summers, J. Kimberley; Till, Rob; Milner, David S.; Atterbury, Robert J.; Stroud, Amy; Capeness, Michael J.; Gray, Stephanie; Leidenroth, Andreas; Lambert, Carey; Connerton, Ian; Twycross, Jamie; Baker, Michelle; Tyson, Jess; Kreft, Jan Ulrich; Sockett, R. Elizabeth

Abstract

Copyright © 2020 Hobley et al. Bacteria are preyed upon by diverse microbial predators, including bacteriophage and predatory bacteria, such as Bdellovibrio bacteriovorus. While bacteriophage are used as antimicrobial therapies in Eastern Europe and are being applied for compassionate use in the United States, predatory bacteria are only just beginning to reveal their potential therapeutic uses. However, predation by either predator type can falter due to different adaptations arising in the prey bacteria. When testing poultry farm wastewater for novel Bdellovibrio isolates on Escherichia coli prey lawns, individual composite plaques were isolated containing both an RTP (rosette-tailed-phage)-like-phage and a B. bacteriovorus strain and showing central prey lysis and halos of extra lysis. Combining the purified phage with a lab strain of B. bacteriovorus HD100 recapitulated haloed plaques and increased killing of the E. coli prey in liquid culture, showing an effective side-by-side action of these predators compared to their actions alone. Using approximate Bayesian computation to select the best fitting from a variety of different mathematical models demonstrated that the experimental data could be explained only by assuming the existence of three prey phenotypes: (i) sensitive to both predators, (ii) genetically resistant to phage only, and (iii) plastic resistant to B. bacteriovorus only. Although each predator reduces prey availability for the other, high phage numbers did not abolish B. bacteriovorus predation, so both predators are competent to coexist and are causing different selective pressures on the bacterial surface while, in tandem, controlling prey bacterial numbers efficiently. This suggests that combinatorial predator therapy could overcome problems of phage resistance. Importance: With increasing levels of antibiotic resistance, the development of alternative antibacterial therapies is urgently needed. Two potential alternatives are bacteriophage and predatory bacteria. Bacteriophage therapy has been used, but prey/host specificity and the rapid acquisition of bacterial resistance to bacteriophage are practical considerations. Predatory bacteria are of interest due to their broad Gram-negative bacterial prey range and the lack of simple resistance mechanisms. Here, a bacteriophage and a strain of Bdellovibrio bacteriovorus, preyed side by side on a population of E. coli, causing a significantly greater decrease in prey numbers than either alone. Such combinatorial predator therapy may have greater potential than individual predators since prey surface changes selected for by each predator do not protect prey against the other predator.

Citation

Hobley, L., Summers, J. K., Till, R., Milner, D. S., Atterbury, R. J., Stroud, A., Capeness, M. J., Gray, S., Leidenroth, A., Lambert, C., Connerton, I., Twycross, J., Baker, M., Tyson, J., Kreft, J. U., & Sockett, R. E. (2020). Dual predation by bacteriophage and bdellovibrio bacteriovorus can eradicate escherichia coli prey in situations where single predation cannot. Journal of Bacteriology, 202(6), Article e00629-19. https://doi.org/10.1128/JB.00629-19

• Journal Article Type: Article
• Acceptance Date: Dec 17, 2019
• Online Publication Date: Jan 6, 2020
• Publication Date: Jan 6, 2020
• Deposit Date: Jan 9, 2020
• Publicly Available Date: Jan 9, 2020
• Journal: Journal of Bacteriology
• Print ISSN: 0021-9193
• Electronic ISSN: 1098-5530
• Publisher: American Society for Microbiology
• Peer Reviewed: Peer Reviewed
• Volume: 202
• Issue: 6
• Article Number: e00629-19
• DOI: https://doi.org/10.1128/JB.00629-19
• Keywords: Molecular Biology; Microbiology
• Public URL: https://nottingham-repository.worktribe.com/output/3696668
• Publisher URL: https://jb.asm.org/content/early/2020/01/02/JB.00629-19