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Effect of Soybean Oil and Fish Oil on Lipid-Related Transcripts in Subcutaneous Adipose Tissue of Dairy Cows

Vargas-Bello-Pérez, Einar; Bionaz, Massimo; Garrido-Sartore, Macarena; Cancino-Padilla, Nathaly; Morales, María Sol; Romero, Jaime; Leskinen, Heidi; Garnsworthy, Philip C.; Loor, Juan J.

Authors

Einar Vargas-Bello-Pérez

Massimo Bionaz

Macarena Garrido-Sartore

Nathaly Cancino-Padilla

María Sol Morales

Jaime Romero

Heidi Leskinen

Juan J. Loor



Abstract

The objective of this study was to determine the effect of long-term supplementation of unsaturated oil on lipid metabolism and transcription of genes involved in lipid metabolism in subcutaneous adipose tissue (SAT) of mid-lactating dairy cows. The objective was achieved by supplementing dairy cows with soybean oil (SO; high in linoleic acid) or fish oil (FO; high in EPA and DHA) for 63 days (nine weeks). Cows were fed a control diet with no added lipid, or diets containing SO or FO (n = 5 cows/group). At the onset of the experiment (day 0) and on days 21, 42, and 63 of supplementation, blood and SAT samples were collected from each animal. Oil supplementation increased cholesterol and NEFA in plasma, with a greater effect of SO compared to FO. Concentration of BUN was lower in SO compared to control and FO at the end of the trial. Transcription of few genes was affected by dietary lipids: FABP4 had lowest expression in FO followed by SO and control. ACACA and FASN had higher expression in FO. Transcription of SCAP was higher but expression of INSIG1 was lower in SO. Overall, results revealed that compared to control, SO and FO had lipogenic effect in SAT.

Citation

Vargas-Bello-Pérez, E., Bionaz, M., Garrido-Sartore, M., Cancino-Padilla, N., Morales, M. S., Romero, J., …Loor, J. J. (2019). Effect of Soybean Oil and Fish Oil on Lipid-Related Transcripts in Subcutaneous Adipose Tissue of Dairy Cows. Animals, 10(1), https://doi.org/10.3390/ani10010054

Journal Article Type Article
Acceptance Date Dec 23, 2019
Online Publication Date Dec 26, 2019
Publication Date Dec 26, 2019
Deposit Date Feb 11, 2020
Publicly Available Date Nov 30, -0001
Journal Animals
Electronic ISSN 2076-2615
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 10
Issue 1
Article Number 54
DOI https://doi.org/10.3390/ani10010054
Keywords fat supplementation; transcriptomic; subcutaneous adipose tissue; lactating cows
Public URL https://nottingham-repository.worktribe.com/output/3637515
Publisher URL https://www.mdpi.com/2076-2615/10/1/54

Files

E ect of Soybean Oil and Fish Oil (1.7 Mb)
PDF

Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/





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