Thamali Ayagama
Compartmentalization proteomics revealed endolysosomal protein network changes in a goat model of atrial fibrillation
Ayagama, Thamali; Charles, Philip D.; Bose, Samuel J.; Boland, Barry; Priestman, David A.; Aston, Daniel; Berridge, Georgina; Fisher, Roman; Cribbs, Adam P.; Song, Qianqian; Mirams, Gary R.; Amponsah, Kwabena; Heather, Lisa; Galione, Antony; Herring, Neil; Kramer, Holger; Capel, Rebecca A.; Platt, Frances M.; Schotten, Ulrich; Verheule, Sander; Burton, Rebecca A.B.
Authors
Philip D. Charles
Samuel J. Bose
Barry Boland
David A. Priestman
Daniel Aston
Georgina Berridge
Roman Fisher
Adam P. Cribbs
Qianqian Song
Prof. GARY MIRAMS GARY.MIRAMS@NOTTINGHAM.AC.UK
Professor of Mathematical Biology
Kwabena Amponsah
Lisa Heather
Antony Galione
Neil Herring
Holger Kramer
Rebecca A. Capel
Frances M. Platt
Ulrich Schotten
Sander Verheule
Rebecca A.B. Burton
Abstract
Endolysosomes (EL) are known for their role in regulating both intracellular trafficking and proteostasis. EL facilitate the elimination of damaged membranes, protein aggregates, membranous organelles and play an important role in calcium signaling. The specific role of EL in cardiac atrial fibrillation (AF) is not well understood. We isolated atrial EL organelles from AF goat biopsies and conducted a comprehensive integrated omics analysis to study the EL-specific proteins and pathways. We also performed electron tomography, protein and enzyme assays on these biopsies. Our results revealed the upregulation of the AMPK pathway and the expression of EL-specific proteins that were not found in whole tissue lysates, including GAA, DYNLRB1, CLTB, SIRT3, CCT2, and muscle-specific HSPB2. We also observed structural anomalies, such as autophagic-vacuole formation, irregularly shaped mitochondria, and glycogen deposition. Our results provide molecular information suggesting EL play a role in AF disease process over extended time frames.
Citation
Ayagama, T., Charles, P. D., Bose, S. J., Boland, B., Priestman, D. A., Aston, D., …Burton, R. A. (2024). Compartmentalization proteomics revealed endolysosomal protein network changes in a goat model of atrial fibrillation. iScience, 27(6), Article 109609. https://doi.org/10.1016/j.isci.2024.109609
Journal Article Type | Article |
---|---|
Acceptance Date | Mar 25, 2024 |
Online Publication Date | Mar 28, 2024 |
Publication Date | Jun 21, 2024 |
Deposit Date | Jun 11, 2024 |
Publicly Available Date | Jun 12, 2024 |
Journal | iScience |
Electronic ISSN | 2589-0042 |
Publisher | Cell Press |
Peer Reviewed | Peer Reviewed |
Volume | 27 |
Issue | 6 |
Article Number | 109609 |
DOI | https://doi.org/10.1016/j.isci.2024.109609 |
Public URL | https://nottingham-repository.worktribe.com/output/33282097 |
Publisher URL | https://www.sciencedirect.com/science/article/pii/S2589004224008319 |
Additional Information | This article is maintained by: Elsevier; Article Title: Compartmentalization proteomics revealed endolysosomal protein network changes in a goat model of atrial fibrillation; Journal Title: iScience; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.isci.2024.109609; Content Type: article; Copyright: © 2024 The Author(s). Published by Elsevier Inc. |
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