Structure of a low-population binding intermediate in protein-RNA recognition

Borkar, Aditi N.; Bardaro, Michael F.; Camilloni, Carlo; Aprile, Francesco A.; Varani, Gabriele; Vendruscolo, Michele

Abstract

All biochemical reactions in living organisms require molecular recognition events. In particular, the interactions between protein and RNA molecules are crucial in the regulation of gene expression. However, the transient nature of the conformations populated during the recognition process has prevented a detailed characterization of the mechanisms by which these interactions take place. To address this problem, we report a high-resolution structure of an intermediate state in protein-RNA recognition. We determined this structure by using NMR measurements as ensemble-averaged structural restraints in metadynamics simulations, and validated it by performing a structure-based design of two mutants with rationally modified binding rates.

Citation

Borkar, A. N., Bardaro, M. F., Camilloni, C., Aprile, F. A., Varani, G., & Vendruscolo, M. (2016). Structure of a low-population binding intermediate in protein-RNA recognition. Proceedings of the National Academy of Sciences, 113(26), 7171-7176. https://doi.org/10.1073/pnas.1521349113

• Journal Article Type: Article
• Acceptance Date: Apr 29, 2016
• Online Publication Date: Jun 10, 2016
• Publication Date: Jun 28, 2016
• Deposit Date: Oct 1, 2020
• Journal: Proceedings of the National Academy of Sciences
• Print ISSN: 0027-8424
• Electronic ISSN: 1091-6490
• Publisher: National Academy of Sciences
• Peer Reviewed: Peer Reviewed
• Volume: 113
• Issue: 26
• Pages: 7171-7176
• DOI: https://doi.org/10.1073/pnas.1521349113
• Keywords: Multidisciplinary
• Public URL: https://nottingham-repository.worktribe.com/output/3291339