DWT1/DWL2 act together with OsPIP5K1 to regulate plant uniform growth in rice

Fang, Fang; Ye, Shiwei; Tang, Jingyao; Bennett, Malcolm J.; Liang, Wanqi

Abstract

• Uniform growth of the main shoot and tillers significantly influences rice plant architecture and grain yield. The WUSCHEL‐related homeobox transcription factor DWT1 is a key regulator of this important agronomic trait, disruption of which causes enhanced main shoot dominance and tiller dwarfism by an unknown mechanism.
• Here, we have used yeast‐two‐hybrid screening to identify OsPIP5K1, a member of the rice phosphatidylinositol‐4‐phosphate 5‐kinase family, as a protein that interacts with DWT1. Cytological analyses confirmed that DWT1 induces accumulation of OsPIP5K1 and its product PI(4,5)P2, a phosphoinositide secondary messenger, in nuclear bodies.
• Mutation of OsPIP5K1 compounds the dwarf dwt1 phenotype but abolishes the main shoot dominance. Conversely, overexpression of OsPIP5K1 partially rescues dwt1 developmental defects. Furthermore, we showed that DWL2, the homologue of DWT1, is also able to interact with OsPIP5K1 and shares partial functional redundancy with DWT1 in controlling rice uniformity.
• Overall, our data suggest that nuclear localised OsPIP5K1 acts with DWT1 and/or DWL2 to coordinate the uniform growth of rice shoots, likely to be through nuclear phosphoinositide signals, and provides insights into the regulation of rice uniformity via a largely unexplored plant nuclear signalling pathway.

Citation

Fang, F., Ye, S., Tang, J., Bennett, M. J., & Liang, W. (2020). DWT1/DWL2 act together with OsPIP5K1 to regulate plant uniform growth in rice. New Phytologist, 225(3), 1234-1246. https://doi.org/10.1111/nph.16216

• Journal Article Type: Article
• Acceptance Date: Sep 15, 2019
• Online Publication Date: Sep 24, 2019
• Publication Date: 2020-02
• Deposit Date: Nov 13, 2019
• Publicly Available Date: Sep 25, 2020
• Journal: New Phytologist
• Print ISSN: 0028-646X
• Electronic ISSN: 1469-8137
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 225
• Issue: 3
• Pages: 1234-1246
• DOI: https://doi.org/10.1111/nph.16216
• Keywords: DWT1, DWL2, Nuclear signalling, Phosphatidylinositol-4-phosphate 5-kinase, Phosphoinositide, PIP5K, Plant uniformity, WOX
• Public URL: https://nottingham-repository.worktribe.com/output/3257825
• Publisher URL: https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.16216
• Additional Information: "This is the peer reviewed version of the following article: Fang, F. , Ye, S. , Tang, J. , Bennett, M. J. and Liang, W. (2019), DWT1/DWL2 act together with OsPIP5K1 to regulate plant uniform growth in rice. New Phytol. doi:10.1111/nph.16216, which has been published in final form at https://doi.org/10.1111/nph.16216; . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
• Contract Date: Nov 13, 2019