The impact of CWP and DAN gene-encoded mannoproteins on cell wall thickness under aerobic and anaerobic conditions

Lawrence, Stephen J.; Smart, Katherine A.

Abstract

CWP and DAN gene-encoded mannoproteins are downregulated and up-regulated, respectively, during adaptation to anaerobic growth, contributing to the remodeling of the yeast cell wall. The impact of the absence of these mannoproteins on cell wall thickness was assessed under aerobic and CO2-induced anaerobic conditions. Zymolyase digestion was used to detect the impact of deletion of DAN and CWP genes on the integrity of the cell wall structure. During aerobic incubation, deletion of the CWP1 gene decreased the thickness of the cell wall, whereas deletion of DAN genes did not affect the thickness of the yeast cell wall. Under CO2-induced anaerobic conditions, deletion of DAN2 and DAN3 decreased the thickness of the cell wall, whereas deletion of CWP1, CWP2, DAN1, and DAN4 did not. It is suggested that dan2p and dan3p play a role in determining cell wall thickness during CO2-induced anaerobiosis by bridging and spacing mannoproteins at the cell surface and, thus, facilitating the uptake of nutrients by yeast during fermentation.

Citation

Lawrence, S. J., & Smart, K. A. (2011). The impact of CWP and DAN gene-encoded mannoproteins on cell wall thickness under aerobic and anaerobic conditions. Journal of The American Society of Brewing Chemists, 69(3), 139-143. https://doi.org/10.1094/ASBCJ-2011-0527-01

• Journal Article Type: Article
• Online Publication Date: Feb 5, 2018
• Publication Date: Aug 19, 2011
• Deposit Date: Jul 2, 2024
• Journal: Journal of the American Society of Brewing Chemists
• Print ISSN: 0361-0470
• Electronic ISSN: 1943-7854
• Publisher: Taylor & Francis
• Peer Reviewed: Peer Reviewed
• Volume: 69
• Issue: 3
• Pages: 139-143
• DOI: https://doi.org/10.1094/ASBCJ-2011-0527-01
• Keywords: Anaerobic, Cell wall, DAN genes, Saccharomyces cerevisiae
• Public URL: https://nottingham-repository.worktribe.com/output/3223514
• Publisher URL: https://www.tandfonline.com/doi/abs/10.1094/ASBCJ-2011-0527-01