Krishna Samanta
Ca²⁺ Channel Re-localization to Plasma-Membrane Microdomains Strengthens Activation of Ca2+-Dependent Nuclear Gene Expression
Samanta, Krishna; Kar, Pulak; Mirams, Gary R.; Parekh, Anant B.
Authors
Pulak Kar
Professor GARY MIRAMS GARY.MIRAMS@NOTTINGHAM.AC.UK
PROFESSOR OF MATHEMATICAL BIOLOGY
Anant B. Parekh
Abstract
In polarized cells or cells with complex geometry, clustering of plasma-membrane (PM) ion channels is an effective mechanism for eliciting spatially restricted signals. However, channel clustering is also seen in cells with relatively simple topology, suggesting it fulfills a more fundamental role in cell biology than simply orchestrating compartmentalized responses. Here, we have compared the ability of store-operated Ca2+ release-activated Ca2+ (CRAC) channels confined to PM microdomains with a similar number of dispersed CRAC channels to activate transcription factors, which subsequently increase nuclear gene expression. For similar levels of channel activity, we find that channel confinement is considerably more effective in stimulating gene expression. Our results identify a long-range signaling advantage to the tight evolutionary conservation of channel clustering and reveal that CRAC channel aggregation increases the strength, fidelity, and reliability of the general process of excitation-transcription coupling.
Citation
Samanta, K., Kar, P., Mirams, G. R., & Parekh, A. B. (2015). Ca²⁺ Channel Re-localization to Plasma-Membrane Microdomains Strengthens Activation of Ca2+-Dependent Nuclear Gene Expression. Cell Reports, 12(2), 203-216. https://doi.org/10.1016/j.celrep.2015.06.018
Journal Article Type | Article |
---|---|
Acceptance Date | Jun 4, 2015 |
Publication Date | Jul 14, 2015 |
Deposit Date | Jan 14, 2020 |
Publicly Available Date | Feb 12, 2020 |
Journal | Cell Reports |
Print ISSN | 2211-1247 |
Electronic ISSN | 2211-1247 |
Publisher | Cell Press |
Peer Reviewed | Peer Reviewed |
Volume | 12 |
Issue | 2 |
Pages | 203-216 |
DOI | https://doi.org/10.1016/j.celrep.2015.06.018 |
Public URL | https://nottingham-repository.worktribe.com/output/3217458 |
Publisher URL | https://www.sciencedirect.com/science/article/pii/S2211124715006166 |
Files
Ca2+ channel
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Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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