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Ca²⁺ Channel Re-localization to Plasma-Membrane Microdomains Strengthens Activation of Ca2+-Dependent Nuclear Gene Expression

Samanta, Krishna; Kar, Pulak; Mirams, Gary R.; Parekh, Anant B.

Ca²⁺ Channel Re-localization to Plasma-Membrane Microdomains Strengthens Activation of Ca2+-Dependent Nuclear Gene Expression Thumbnail


Authors

Krishna Samanta

Pulak Kar

Anant B. Parekh



Abstract

In polarized cells or cells with complex geometry, clustering of plasma-membrane (PM) ion channels is an effective mechanism for eliciting spatially restricted signals. However, channel clustering is also seen in cells with relatively simple topology, suggesting it fulfills a more fundamental role in cell biology than simply orchestrating compartmentalized responses. Here, we have compared the ability of store-operated Ca2+ release-activated Ca2+ (CRAC) channels confined to PM microdomains with a similar number of dispersed CRAC channels to activate transcription factors, which subsequently increase nuclear gene expression. For similar levels of channel activity, we find that channel confinement is considerably more effective in stimulating gene expression. Our results identify a long-range signaling advantage to the tight evolutionary conservation of channel clustering and reveal that CRAC channel aggregation increases the strength, fidelity, and reliability of the general process of excitation-transcription coupling.

Citation

Samanta, K., Kar, P., Mirams, G. R., & Parekh, A. B. (2015). Ca²⁺ Channel Re-localization to Plasma-Membrane Microdomains Strengthens Activation of Ca2+-Dependent Nuclear Gene Expression. Cell Reports, 12(2), 203-216. https://doi.org/10.1016/j.celrep.2015.06.018

Journal Article Type Article
Acceptance Date Jun 4, 2015
Publication Date Jul 14, 2015
Deposit Date Jan 14, 2020
Publicly Available Date Feb 12, 2020
Journal Cell Reports
Print ISSN 2211-1247
Electronic ISSN 2211-1247
Publisher Cell Press
Peer Reviewed Peer Reviewed
Volume 12
Issue 2
Pages 203-216
DOI https://doi.org/10.1016/j.celrep.2015.06.018
Public URL https://nottingham-repository.worktribe.com/output/3217458
Publisher URL https://www.sciencedirect.com/science/article/pii/S2211124715006166

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