Extracting DNA from within intact foraminiferal shells

Seears, Heidi A.; Wade, Christopher M.

Abstract

Here we present an evaluation of several methods for extracting DNA from within intact foraminiferal shells. We tested 12 lysis buffers under carefully optimised incubation conditions, evaluating their success in terms of efficiency in evacuating cellular material, crude DNA yield, PCR success, and final shell integrity. A number of the buffers tested produced excellent results. The most successful method utilized a lysis buffer containing Sodium N-lauroyl sarcosine, Guanidinium isothiocyanate, Isopropanol, TRIS and NaCl, incubated at 75. °C for 24. h, followed by chloroform extraction and ethanol precipitation. High yields of DNA were produced, with no signs of PCR inhibition, and the foraminiferal shells were left completely intact. Retaining the shell of individual specimens presents a significant advance, allowing for direct comparisons between shell morphology and genotype data, which could greatly enhance the utility of foraminifera as palaeoproxies of past climate change. © 2014 Elsevier B.V.

Citation

Seears, H. A., & Wade, C. M. (2014). Extracting DNA from within intact foraminiferal shells. Marine Micropaleontology, 109, 46-53. https://doi.org/10.1016/j.marmicro.2014.04.001

• Journal Article Type: Article
• Acceptance Date: Apr 3, 2014
• Online Publication Date: Apr 13, 2014
• Publication Date: May 1, 2014
• Deposit Date: Aug 8, 2022
• Journal: Marine Micropaleontology
• Print ISSN: 0377-8398
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 109
• Pages: 46-53
• DOI: https://doi.org/10.1016/j.marmicro.2014.04.001
• Public URL: https://nottingham-repository.worktribe.com/output/3189003
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0377839814000425?via%3Dihub