Liposome entrapment and immunogenic studies of a synthetic lipophilic multiple antigenic peptide bearing VP1 and VP3 domains of the hepatitis A virus: A robust method for vaccine design

Haro, Isabel; Pérez, Silvia; García, Mónica; Chan, Weng C.; Ercilla, Guadalupe

Abstract

Multiple antigen peptides (MAP) have been demonstrated to be efficient immunological reagents for the induction of immune responses to a variety of infectious agents. Several peptide domains of the hepatitis A virus (HAV) capsid proteins, mainly VP1 and VP3, are the immunodominant targets for a protective antibody response. In the present study we analyse the immunogenic properties of a tetrameric heterogeneous palmitoyl-derivatised MAP containing two defined HAV peptide sequences, VP1(11-25) and VP3(102-121), in rabbits immunised with either Freund's adjuvant or multilamellar liposomes. The immune response was evaluated with a specific enzyme immunoassay using MAP[VP1+VP3], VP1 and VP3 as targets. The avidity of the immune response was measured by a non-competitive enzyme-linked immunosorbent assay and by the surface plasmon resonance technology. Antisera raised against the lipo-MAP peptide entrapped in liposomes demonstrated high avidity of binding with affinity rate constants approximately one order of magnitude greater than those obtained with the Freund's protocol. © 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

Citation

Haro, I., Pérez, S., García, M., Chan, W. C., & Ercilla, G. (2003). Liposome entrapment and immunogenic studies of a synthetic lipophilic multiple antigenic peptide bearing VP1 and VP3 domains of the hepatitis A virus: A robust method for vaccine design. FEBS Letters, 540(1-3), 133-140. https://doi.org/10.1016/S0014-5793%2803%2900249-7

• Journal Article Type: Article
• Acceptance Date: Feb 25, 2003
• Online Publication Date: Mar 14, 2003
• Publication Date: Apr 10, 2003
• Deposit Date: May 12, 2023
• Journal: FEBS Letters
• Print ISSN: 0014-5793
• Electronic ISSN: 1873-3468
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 540
• Issue: 1-3
• Pages: 133-140
• DOI: https://doi.org/10.1016/S0014-5793%2803%2900249-7
• Public URL: https://nottingham-repository.worktribe.com/output/3137770
• Publisher URL: https://febs.onlinelibrary.wiley.com/doi/full/10.1016/S0014-5793%2803%2900249-7