Effects of fatty acids on skeletal muscle cell differentiation in vitro

Hurley, Matthew S.; Flux, Claire; Salter, Andrew M.; Brameld, John M.

Abstract

Previous studies have shown stimulatory effects of linoleic acid (LA, C18:2) on differentiation of rat muscle cells in culture (Allen et al. 1985), but there appears to be little investigation of the effects of other fatty acids. The present study therefore compared the effects of different fatty acids on muscle cell differentiation in vitro. L6 myoblasts were cultured (Dulbecco's Modified Eagles Medium + 10 % fetal calf serum) in six-well plates until 80 % confluent (day 0). Cells were then either harvested or the medium switched to differentiation medium (Dulbecco's Modified Eagles Medium + 2 % horse serum), supplemented with fatty acid or drug treatments. Cells were harvested on days 0-5 and assayed for creatine kinase (CK), protein and DNA contents, to give a measure of differentiation (CK/DNA). Initial studies indicated a stimulatory effect of the cis9,-trans11 (c9,t11) isomer of conjugated linoleic acid (CLA) relative to control. By contrast, the trans10,cis12 (t10,c12) isomer of CLA inhibited differentiation. Further experiments indicated that inhibition of differentiation by the t10,c12 CLA isomer was dose-dependent (up to 200 μM) and may be via increased cell proliferation. LA and c9,t11 CLA stimulated differentiation at low concentrations (up to 50 μM), but inhibited differentiation at high concentrations (200 μM). In contrast, oleic acid stimulated differentiation at all concentrations, whereas the saturated fatty acid, palmitic acid, had no effect. The mechanism appeared not to involve either peroxisome proliferator-activated receptors α or γ. The data suggest that only unsaturated fatty acids have an effect and the presence or absence of a cis-9 double bond may be important. © The Authors 2006.

Citation

Hurley, M. S., Flux, C., Salter, A. M., & Brameld, J. M. (2006). Effects of fatty acids on skeletal muscle cell differentiation in vitro. British Journal of Nutrition, 95(3), 623-630. https://doi.org/10.1079/BJN20051711

• Journal Article Type: Article
• Acceptance Date: Nov 28, 2005
• Publication Date: Mar 1, 2006
• Deposit Date: Feb 9, 2021
• Journal: British Journal of Nutrition
• Print ISSN: 0007-1145
• Electronic ISSN: 1475-2662
• Publisher: Cambridge University Press (CUP)
• Peer Reviewed: Peer Reviewed
• Volume: 95
• Issue: 3
• Pages: 623-630
• DOI: https://doi.org/10.1079/BJN20051711
• Public URL: https://nottingham-repository.worktribe.com/output/3135945