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Identification and characterization of novel antigenic vaccine candidates of Actinobacillus pleuropneumoniae

Oldfield, Neil J.; Donovan, Elizabeth A.; Worrall, Kathryn E.; Wooldridge, Karl G.; Langford, Paul R.; Rycroft, Andrew N.; Ala'Aldeen, Dlawer A.A.

Authors

Elizabeth A. Donovan

Kathryn E. Worrall

Paul R. Langford

Andrew N. Rycroft

Dlawer A.A. Ala'Aldeen



Abstract

Actinobacillus pleuropneumoniae is an important respiratory pathogen of swine, for which there is no highly effective vaccine. A phage expression library of the A. pleuropneumoniae genome was constructed and screened to identify potential vaccine components. Open reading frames within immuno-reactive phage were analyzed in silico to identify conserved outer membrane proteins. Four ORFs, named comL, lolB, lppC and ompA were chosen for further study. The four encoded proteins were shown experimentally to be antigenic, highly conserved, outer membrane, in vivo-expressed proteins. In pig protection studies, none of the proteins was individually capable of protecting pigs from colonization and infection with the homologous A. pleuropneumoniae strain, despite a detectable specific antibody response being induced.

Citation

Oldfield, N. J., Donovan, E. A., Worrall, K. E., Wooldridge, K. G., Langford, P. R., Rycroft, A. N., & Ala'Aldeen, D. A. (2008). Identification and characterization of novel antigenic vaccine candidates of Actinobacillus pleuropneumoniae. Vaccine, 26(16), 1942-1954. https://doi.org/10.1016/j.vaccine.2008.02.022

Journal Article Type Article
Acceptance Date Feb 7, 2008
Online Publication Date Feb 27, 2008
Publication Date Apr 7, 2008
Deposit Date Aug 2, 2022
Journal Vaccine
Print ISSN 0264-410X
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 26
Issue 16
Pages 1942-1954
DOI https://doi.org/10.1016/j.vaccine.2008.02.022
Public URL https://nottingham-repository.worktribe.com/output/3129918
Publisher URL https://www.sciencedirect.com/science/article/pii/S0264410X08001643?via%3Dihub