Alisa A. Shaimardanova
Production and Application of Multicistronic Constructs for Various Human Disease Therapies
Shaimardanova, Alisa A.; Kitaeva, Kristina V.; Abdrakhmanova, Ilmira I.; Chernov, Vladislav M.; Rutland, Catrin S.; Rizvanov, Albert A.; Chulpanova, Daria S.; Solovyeva, Valeriya V.
Authors
Kristina V. Kitaeva
Ilmira I. Abdrakhmanova
Vladislav M. Chernov
CATRIN RUTLAND CATRIN.RUTLAND@NOTTINGHAM.AC.UK
Professor of Molecular Medicine
Albert A. Rizvanov
Daria S. Chulpanova
Valeriya V. Solovyeva
Abstract
The development of multicistronic vectors has opened up new opportunities to address the fundamental issues of molecular and cellular biology related to the need for the simultaneous delivery and joint expression of several genes. To date, the examples of the successful use of multicistronic vectors have been described for the development of new methods of treatment of various human diseases, including cardiovascular, oncological, metabolic, autoimmune, and neurodegenerative disorders. The safety and effectiveness of the joint delivery of therapeutic genes in multicistronic vectors based on the internal ribosome entry site (IRES) and self-cleaving 2A peptides have been shown in both in vitro and in vivo experiments as well as in clinical trials. Co-expression of several genes in one vector has also been used to create animal models of various inherited diseases which are caused by mutations in several genes. Multicistronic vectors provide expression of all mutant genes, which allows the most complete mimicking disease pathogenesis. This review comprehensively discusses multicistronic vectors based on IRES nucleotide sequence and self-cleaving 2A peptides, including its features and possible application for the treatment and modeling of various human diseases.
Citation
Shaimardanova, A. A., Kitaeva, K. V., Abdrakhmanova, I. I., Chernov, V. M., Rutland, C. S., Rizvanov, A. A., …Solovyeva, V. V. (2019). Production and Application of Multicistronic Constructs for Various Human Disease Therapies. Pharmaceutics, 11, 580. https://doi.org/10.3390/pharmaceutics11110580
Journal Article Type | Article |
---|---|
Acceptance Date | Nov 3, 2019 |
Online Publication Date | Nov 6, 2019 |
Publication Date | Nov 6, 2019 |
Deposit Date | Nov 8, 2019 |
Publicly Available Date | Nov 8, 2019 |
Journal | Pharmaceutics |
Electronic ISSN | 1999-4923 |
Publisher | MDPI |
Peer Reviewed | Peer Reviewed |
Volume | 11 |
Pages | 580 |
DOI | https://doi.org/10.3390/pharmaceutics11110580 |
Keywords | 2A peptides; IRES; Multicistronic constructs; Gene therapy; Neurodegenerative diseases; Metabolic diseases; Cancer; Autoimmune diseases; Cardiovascular diseases |
Public URL | https://nottingham-repository.worktribe.com/output/3083255 |
Publisher URL | https://www.mdpi.com/1999-4923/11/11/580 |
Contract Date | Nov 8, 2019 |
Files
Production and Application of Multicistronic constructs
(725 Kb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
You might also like
Novel Insights Into the Architecture of Macro and Microstructures in Cattle Ossa Cordis
(2024)
Journal Article
Changes in Immune Response during Pig Gestation with a Focus on Cytokines
(2024)
Journal Article
Downloadable Citations
About Repository@Nottingham
Administrator e-mail: discovery-access-systems@nottingham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search