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Alcoholic fermentation of thermochemical and biological hydrolysates derived from Miscanthus biomass by Clostridium acetobutylicum ATCC 824

Raut, Mahendra P.; Pham, Trong K.; Gomez, Leonardo D.; Dimitriou, Ioanna; Wright, Phillip C.

Authors

Mahendra P. Raut

Trong K. Pham

Leonardo D. Gomez

Phillip C. Wright



Abstract

© 2019 This laboratory scale study aims to demonstrate the effectiveness of thermochemical and biological saccharification of Miscanthus giganteus (MG) for generation of fermentable saccharides and its subsequent fermentation into solvents i.e. acetone, ethanol and butanol (ABE) using Clostridium acetobutylicum ATCC 824. Saccharide hydrolysates were derived from MG by thermochemical (water, acid and alkali at 130 °C) and biological saccharification (Fibrobacter succinogenes S85) processes and were subjected to batch fermentation for 120 h using C. acetobutylicum ATCC 824. At the end of fermentation of thermochemically-derived hydrolysates, 742 g m−3 of saccharides from water treatment, 9572 g m−3 of saccharides from acid treatment and 4054 g m−3 of saccharides from alkali treatment were fermented and yielded 0.045, 0.0069 and 0.01 g g−1 of total solvents, respectively. Similarly, at the end of fermentation of biological hydrolysate (using F. succinogenes), 2504 g m−3 of saccharides was fermented and yielded 0.091 g g−1 of total solvents. The highest yield of total solvents was achieved by water (thermochemical) and biological saccharification of MG using C. acetobutylicum. Whereas, acid and alkali-treated hydrolysates showed lower yields of solvents presumably due to production of inhibitory compounds during saccharification. Compared to thermochemical saccharification, biological saccharification using F. succinogenes is a promising approach since it yielded the highest amount of solvents whilst being eco-friendly. Our future studies will focus on optimisation of biological saccharification (using F. succinogenes) and sequential co-culture fermentation (using C. acetobutylicum). The development of alternative consolidated bioprocessing approach using biological saccharification will contribute towards making lignocellulosic biofuels a reality.

Citation

Raut, M. P., Pham, T. K., Gomez, L. D., Dimitriou, I., & Wright, P. C. (2019). Alcoholic fermentation of thermochemical and biological hydrolysates derived from Miscanthus biomass by Clostridium acetobutylicum ATCC 824. Biomass and Bioenergy, 130, https://doi.org/10.1016/j.biombioe.2019.105382

Journal Article Type Article
Acceptance Date Sep 18, 2019
Online Publication Date Sep 26, 2019
Publication Date Nov 1, 2019
Deposit Date Nov 13, 2019
Publicly Available Date Sep 27, 2020
Journal Biomass and Bioenergy
Print ISSN 0961-9534
Electronic ISSN 1873-2909
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 130
Article Number 105382
DOI https://doi.org/10.1016/j.biombioe.2019.105382
Keywords Agronomy and Crop Science; Renewable Energy, Sustainability and the Environment; Waste Management and Disposal; Forestry
Public URL https://nottingham-repository.worktribe.com/output/3019966
Additional Information This article is maintained by: Elsevier; Article Title: Alcoholic fermentation of thermochemical and biological hydrolysates derived from Miscanthus biomass by Clostridium acetobutylicum ATCC 824; Journal Title: Biomass and Bioenergy; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.biombioe.2019.105382; Content Type: article; Copyright: © 2019 Published by Elsevier Ltd.

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