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Time resolved transient circular dichroism spectroscopy using synchrotron natural polarization

Auvray, François; Dennetiere, David; Giuliani, Alexandre; Jamme, Frédéric; Wien, Frank; Nay, Bastien; Zirah, Séverine; Polack, François; Menneglier, Claude; Lagarde, Bruno; Hirst, Jonathan D.; Réfrégiers, Matthieu

Authors

François Auvray

David Dennetiere

Alexandre Giuliani

Frédéric Jamme

Frank Wien

Bastien Nay

Séverine Zirah

François Polack

Claude Menneglier

Bruno Lagarde

Matthieu Réfrégiers



Abstract

Ultraviolet (UV) synchrotron radiation circular dichroism (SRCD) spectroscopy has made an important contribution to the determination and understanding of the structure of bio-molecules. In this paper, we report an innovative approach that we term time-resolved SRCD (tr-SRCD), which overcomes the limitations of current broadband UV SRCD setups. This technique allows accessing ultrafast time scales (down to nanoseconds), previously measurable only byother methods, such as infrared (IR), nuclear magnetic resonance (NMR), fluorescence and absorbance spectroscopies, and small angle X-ray scattering (SAXS). The tr-SRCD setup takes advantage of the natural polarization of the synchrotron radiation emitted by a bending magnet to record broadband UV CD faster than any current SRCD setup, improving the acquisition speed from 10 mHz to 130Hz and the accessible temporal resolution by several orders of magnitude. We illustrate the new approach by following the isomer concentration changes of an azopeptide after a photoisomerization. This breakthrough in SRCD spectroscopy opens up a wide range of potential applications to the detailed characterization of biological processes, such as protein folding and protein-ligand binding.

Citation

Auvray, F., Dennetiere, D., Giuliani, A., Jamme, F., Wien, F., Nay, B., …Réfrégiers, M. (2019). Time resolved transient circular dichroism spectroscopy using synchrotron natural polarization. Structural Dynamics, 6(5), https://doi.org/10.1063/1.5120346

Journal Article Type Article
Acceptance Date Oct 9, 2019
Online Publication Date Oct 31, 2019
Publication Date 2019-09
Deposit Date Nov 1, 2019
Publicly Available Date Nov 1, 2019
Journal Structural Dynamics
Electronic ISSN 2329-7778
Publisher AIP Publishing
Peer Reviewed Peer Reviewed
Volume 6
Issue 5
Article Number 054307
DOI https://doi.org/10.1063/1.5120346
Public URL https://nottingham-repository.worktribe.com/output/3009945
Publisher URL https://aca.scitation.org/doi/10.1063/1.5120346

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