Time resolved transient circular dichroism spectroscopy using synchrotron natural polarization

Auvray, Fran�ois; Dennetiere, David; Giuliani, Alexandre; Jamme, Fr�d�ric; Wien, Frank; Nay, Bastien; Zirah, S�verine; Polack, Fran�ois; Menneglier, Claude; Lagarde, Bruno; Hirst, Jonathan D.; R�fr�giers, Matthieu

Abstract

Ultraviolet (UV) synchrotron radiation circular dichroism (SRCD) spectroscopy has made an important contribution to the determination and understanding of the structure of bio-molecules. In this paper, we report an innovative approach that we term time-resolved SRCD (tr-SRCD), which overcomes the limitations of current broadband UV SRCD setups. This technique allows accessing ultrafast time scales (down to nanoseconds), previously measurable only byother methods, such as infrared (IR), nuclear magnetic resonance (NMR), fluorescence and absorbance spectroscopies, and small angle X-ray scattering (SAXS). The tr-SRCD setup takes advantage of the natural polarization of the synchrotron radiation emitted by a bending magnet to record broadband UV CD faster than any current SRCD setup, improving the acquisition speed from 10 mHz to 130Hz and the accessible temporal resolution by several orders of magnitude. We illustrate the new approach by following the isomer concentration changes of an azopeptide after a photoisomerization. This breakthrough in SRCD spectroscopy opens up a wide range of potential applications to the detailed characterization of biological processes, such as protein folding and protein-ligand binding.

Citation

Auvray, F., Dennetiere, D., Giuliani, A., Jamme, F., Wien, F., Nay, B., Zirah, S., Polack, F., Menneglier, C., Lagarde, B., Hirst, J. D., & Réfrégiers, M. (2019). Time resolved transient circular dichroism spectroscopy using synchrotron natural polarization. Structural Dynamics, 6(5), Article 054307. https://doi.org/10.1063/1.5120346

• Journal Article Type: Article
• Acceptance Date: Oct 9, 2019
• Online Publication Date: Oct 31, 2019
• Publication Date: 2019-09
• Deposit Date: Nov 1, 2019
• Publicly Available Date: Nov 1, 2019
• Journal: Structural Dynamics
• Electronic ISSN: 2329-7778
• Publisher: American Institute of Physics
• Peer Reviewed: Peer Reviewed
• Volume: 6
• Issue: 5
• Article Number: 054307
• DOI: https://doi.org/10.1063/1.5120346
• Public URL: https://nottingham-repository.worktribe.com/output/3009945
• Publisher URL: https://aca.scitation.org/doi/10.1063/1.5120346
• Contract Date: Nov 1, 2019