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Formulating injectable pastes of porous calcium phosphate glass microspheres for bone regeneration applications

Matamoros-Veloza, Adriana; Hossain, Kazi M. Zakir; Scammell, Brigitte E.; Ahmed, Ifty; Hall, Richard; Kapur, Nikil

Authors

Adriana Matamoros-Veloza

Kazi M. Zakir Hossain

BRIGITTE SCAMMELL b.scammell@nottingham.ac.uk
Dean of The School of Medicine/ professor of Orthopaedic Sciences

Richard Hall

Nikil Kapur



Abstract

Current trends in regenerative medicine treatments for bone repair applications focus on cell-based therapies. These aim to deliver the treatment via a minimally invasive injection to reduce patient trauma and to improve efficacy. This paper describes the injectability of porous calcium phosphate glass microspheres to be used for bone repair based on their formulation, rheology and flow behavior. The use of excipients (xanthan gum, methyl cellulose and carboxyl methyl cellulose) were investigated to improve flow performance. Based on our results, the flow characteristics of the glass microsphere pastes vary according to particle size, surface area, and solid to liquid ratio, as well as the concentration of viscosity modifiers used. The optimal flow characteristics of calcium phosphate glass microsphere pastes was found to contain 40 mg/mL of xanthan gum which increased viscosity whilst providing elastic properties (∼29,000 Pa) at shear rates that mirror the injection process and the resting period post injection, preventing the glass microspheres from both damage and dispersion. It was established that a base formulation must contain 1 g of glass microspheres (60–125 μm in size) per 1 mL of cell culture media, or 0.48 g of glass microspheres of sizes between 125 and 200 μm. Furthermore, the glass microsphere formulations with xanthan gum were readily injectable via a syringe-needle system (3–20 mL, 18G and 14G needles), and have the potential to be utilized as a cell (or other biologics) delivery vehicle for bone regeneration applications.

Citation

Matamoros-Veloza, A., Hossain, K. M. Z., Scammell, B. E., Ahmed, I., Hall, R., & Kapur, N. (2020). Formulating injectable pastes of porous calcium phosphate glass microspheres for bone regeneration applications. Journal of the Mechanical Behavior of Biomedical Materials, 102, https://doi.org/10.1016/j.jmbbm.2019.103489

Journal Article Type Article
Acceptance Date Oct 10, 2019
Online Publication Date Oct 11, 2019
Publication Date 2020-02
Deposit Date Oct 18, 2019
Publicly Available Date Oct 12, 2020
Journal Journal of the Mechanical Behavior of Biomedical Materials
Print ISSN 1751-6161
Electronic ISSN 1878-0180
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 102
Article Number 103489
DOI https://doi.org/10.1016/j.jmbbm.2019.103489
Keywords Mechanics of Materials; Biomaterials; Biomedical Engineering
Public URL https://nottingham-repository.worktribe.com/output/2857307
Publisher URL https://www.sciencedirect.com/science/article/pii/S175161611930935X
Additional Information This article is maintained by: Elsevier; Article Title: Formulating injectable pastes of porous calcium phosphate glass microspheres for bone regeneration applications; Journal Title: Journal of the Mechanical Behavior of Biomedical Materials; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.jmbbm.2019.103489; Content Type: article; Copyright: Crown Copyright © 2019 Published by Elsevier Ltd. All rights reserved.

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