Phosphate glass fibers facilitate proliferation and osteogenesis through Runx2 transcription in murine osteoblastic cells

Lin, Xiao; Li, Meng; Chen, Qiang; Xiao, Yunyun; Gao, Yongguang; Ahmed, Ifty; Li, Hui; Zhang, Kewen; Qiu, Wuxia; Liu, Xianhu; Boccaccini, Aldo R; Qian, Airong

Abstract

Cell-material interactions and compatibility are important aspects of bioactive materials for bone tissue engineering. Phosphate glass fiber (PGF) is an attractive inorganic filler with fibrous structure and tunable composition, which has been widely investigated as a bioactive filler for bone repair applications. However, the interaction of osteoblasts with PGFs has not been widely investigated to elucidate the osteogenic mechanism of PGFs. In this study, different concentrations of short PGFs with interlaced oriented topography were co-cultured with MC3T3-E1 cells for different periods, and the synergistic effects of fiber topography and ionic product of PGFs on osteoblast responses including cell adhesion, spreading, proliferation and osteogenic differentiation were investigated. It was found that osteoblasts were more prone to adhere on PGFs through vinculin protein, leading to enhanced cell proliferation with polygonal cell shape and spreading cellular actin filaments. In addition, osteoblasts incubated on PGF meshes showed enhanced alkaline phosphatase (ALP) activity, extracellular matrix mineralization, and increased expression of osteogenesis-related marker genes, which could be attributed to the Wnt/β-catenin/Runx2 signaling pathway. This study elucidated the possible mechanism of PGF on triggering specific osteoblast behavior, which would be highly beneficial for designing PGF-based bone graft substitutes with excellent osteogenic functions.

Citation

Lin, X., Li, M., Chen, Q., Xiao, Y., Gao, Y., Ahmed, I., …Qian, A. (2020). Phosphate glass fibers facilitate proliferation and osteogenesis through Runx2 transcription in murine osteoblastic cells. Journal of Biomedical Materials Research Part A, 108(2), 316-326. https://doi.org/10.1002/jbm.a.36818

• Journal Article Type: Article
• Acceptance Date: Oct 10, 2019
• Online Publication Date: Oct 19, 2019
• Publication Date: Feb 1, 2020
• Deposit Date: Oct 18, 2019
• Publicly Available Date: Oct 20, 2020
• Journal: Journal of Biomedical Materials Research Part A
• Print ISSN: 1549-3296
• Electronic ISSN: 1552-4965
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 108
• Issue: 2
• Pages: 316-326
• DOI: https://doi.org/10.1002/jbm.a.36818
• Keywords: Cell-material interaction, Phosphate glass fibers, Osteogenesis, Signaling pathway, Runx2
• Public URL: https://nottingham-repository.worktribe.com/output/2856742
• Publisher URL: https://onlinelibrary.wiley.com/doi/abs/10.1002/jbm.a.36818
• Additional Information: This is the peer reviewed version of the following article: Lin, X. , Chen, Q. , Xiao, Y. , Gao, Y. , Ahmed, I. , Li, M. , Li, H. , Zhang, K. , Qiu, W. , Liu, X. , Boccaccini, A. R. and Qian, A. (2019), Phosphate glass fibers facilitate proliferation and osteogenesis through Runx2 transcription in murine osteoblastic cells. J Biomed Mater Res, which has been published in final form at https://doi.org/10.1002/jbm.a.36818 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
• Contract Date: Oct 18, 2019