Untargeted analysis of plasma samples from pre-eclamptic women reveals polar and apolar changes in the metabolome

Sander, Katrin N.; Kim, Dong-Hyun; Ortori, Catherine A.; Warren, Averil Y.; Anyanwagu, Uchenna C.; Hay, Daniel P.; Pipkin, Fiona Broughton; Khan, Raheela N.; Barrett, David A.

Abstract

Introduction
Pre-eclampsia is a hypertensive gestational disorder that affects approximately 5% of all pregnancies.

Objectives
As the pathophysiological processes of pre-eclampsia are still uncertain, the present case–control study explored underlying metabolic processes characterising this disease.

Methods
Maternal peripheral plasma samples were collected from pre-eclamptic (n = 32) and healthy pregnant women (n = 35) in the third trimester. After extraction, high-resolution mass spectrometry-based untargeted metabolomics was used to profile polar and apolar metabolites and the resulting data were analysed via uni- and multivariate statistical approaches.

Results
The study demonstrated that the metabolome undergoes substantial changes in pre-eclamptic women. Amongst the most discriminative metabolites were hydroxyhexacosanoic acid, diacylglycerols, glycerophosphoinositols, nicotinamide adenine dinucleotide metabolites, bile acids and products of amino acid metabolism.

Conclusions
The putatively identified compounds provide sources for novel hypotheses to help understanding of the underlying biochemical pathology of pre-eclampsia.

Citation

Sander, K. N., Kim, D., Ortori, C. A., Warren, A. Y., Anyanwagu, U. C., Hay, D. P., …Barrett, D. A. (2019). Untargeted analysis of plasma samples from pre-eclamptic women reveals polar and apolar changes in the metabolome. Metabolomics, 15, 1-10. https://doi.org/10.1007/s11306-019-1600-8

• Journal Article Type: Article
• Acceptance Date: Sep 25, 2019
• Online Publication Date: Nov 27, 2019
• Publication Date: Dec 31, 2019
• Deposit Date: Oct 17, 2019
• Publicly Available Date: Nov 27, 2019
• Journal: Metabolomics
• Electronic ISSN: 1573-3890
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 15
• Article Number: 157
• Pages: 1-10
• DOI: https://doi.org/10.1007/s11306-019-1600-8
• Keywords: Clinical Biochemistry; Biochemistry; Endocrinology, Diabetes and Metabolism
• Public URL: https://nottingham-repository.worktribe.com/output/2853621
• Publisher URL: https://link.springer.com/article/10.1007/s11306-019-1600-8
• Additional Information: Received: 20 May 2019; Accepted: 27 September 2019; First Online: 27 November 2019; : ; : All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants included in the study.
• Contract Date: Oct 17, 2019