Mitochondrial DNA copy number associates with insulin sensitivity and aerobic capacity, and differs between sedentary, overweight middle-aged males with and without type 2 diabetes

Constantin-Teodosiu, Dumitru; Constantin, Despina; Pelsers, Maurice M.; Verdijk, Lex B.; van Loon, Luc; Greenhaff, Paul

Abstract

Background/objectives: Increased risk of type 2 diabetes mellitus (T2DM) is linked to impaired muscle mitochondrial function and reduced mitochondrial DNA copy number (mtDNAnum). However, studies have failed to control for habitual physical activity levels, which directly influences both mtDNA copy number and insulin sensitivity. We, therefore, examined whether physical conditioning status (maximal oxygen uptake, V̇O2max) was associated with skeletal muscle mitochondrial volume and mtDNAnum, and was predictive of T2DM in overweight, middle-aged men.
Methods: Whole-body physiological (ISI-insulin sensitivity index, HOMA-IR, V̇O2max) and muscle biochemical/molecular (vastus lateralis; mtDNAnum, mitochondrial and glycolytic enzymes activity, lipid content and markers of lipid peroxidation) measurements were performed in 3 groups of overweight, middle-aged male volunteers (n=10 per group): sedentary T2DM (ST2DM); sedentary control (SC) and non-sedentary control (NSC), who differed in aerobic capacity (ST2DM

Citation

Constantin-Teodosiu, D., Constantin, D., Pelsers, M. M., Verdijk, L. B., van Loon, L., & Greenhaff, P. (2019). Mitochondrial DNA copy number associates with insulin sensitivity and aerobic capacity, and differs between sedentary, overweight middle-aged males with and without type 2 diabetes. International Journal of Obesity, https://doi.org/10.1038/s41366-019-0473-2

• Journal Article Type: Article
• Acceptance Date: Sep 30, 2019
• Online Publication Date: Oct 22, 2019
• Publication Date: Oct 22, 2019
• Deposit Date: Oct 17, 2019
• Publicly Available Date: Apr 23, 2020
• Journal: International Journal of Obesity
• Print ISSN: 0307-0565
• Electronic ISSN: 1476-5497
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• DOI: https://doi.org/10.1038/s41366-019-0473-2
• Public URL: https://nottingham-repository.worktribe.com/output/2852020
• Publisher URL: https://www.nature.com/articles/s41366-019-0473-2
• Contract Date: Oct 17, 2019