Expression of polymeric immunoglobulin receptor (PIGR) and the effect of PIGR overexpression on breast cancer cells

Asanprakit, Wichitra; Lobo, Dileep N.; Eremin, Oleg; Bennett, Andrew J

Abstract

Polymeric immunoglobulin receptor (PIGR) has a major role in mucosal immunity as a transporter of polymeric immunoglobulin across the epithelial cells. The aim of this study was to determine the effect of PIGR on cellular behaviours and chemo-sensitivity of MCF7 and MDA-MB468 breast cancer cell lines. Basal levels of PIGR mRNA and protein expression in MCF7 and MDA-MB468 cells were evaluated by real time quantitative polymerase chain reaction and Western blotting, respectively. MCF7/PIGR and MDA-MB468/PIGR stable cell lines, overexpressing the PIGR gene, were generated using a lentiviral vector with tetracycline dependent induction of expression. Cell viability, cell proliferation and chemo-sensitivity of PIGR transfected cells were evaluated and compared with un-transfected cells to determine the effect of PIGR overexpression on cell phenotype. The levels of PIGR mRNA and protein expression were significantly higher in MDA-MB468 cells than in MCF7 cells (380-fold, p < 0.0001). However, the differential expression of PIGR in these two cell lines did not lead to significant differences in chemosensitivity. Viral overexpression of PIGR was also not found to change any of the parameters measured in either cell line. PIGR per se did not affect cellular behaviours and chemosensitivity of these breast cancer cell lines.

Citation

Asanprakit, W., Lobo, D. N., Eremin, O., & Bennett, A. J. (2023). Expression of polymeric immunoglobulin receptor (PIGR) and the effect of PIGR overexpression on breast cancer cells. Scientific Reports, 13(1), Article 16606. https://doi.org/10.1038/s41598-023-43946-6

• Journal Article Type: Article
• Acceptance Date: Sep 30, 2023
• Online Publication Date: Oct 3, 2023
• Publication Date: 2023
• Deposit Date: Oct 5, 2023
• Publicly Available Date: Oct 5, 2023
• Journal: Scientific Reports
• Electronic ISSN: 2045-2322
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 13
• Issue: 1
• Article Number: 16606
• DOI: https://doi.org/10.1038/s41598-023-43946-6
• Keywords: Cell Line, Epithelial Cells, Humans, Breast Neoplasms, Receptors, Polymeric Immunoglobulin, RNA, Messenger, Female
• Public URL: https://nottingham-repository.worktribe.com/output/25679365
• Publisher URL: https://www.nature.com/articles/s41598-023-43946-6
• PMID: 37789066