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In vitro determination of the protein quality of maize varieties cultivated in Malawi using the INFOGEST digestion method.

Muleya, Molly; Li, Dongfang; Chiutsi-Phiri, Gabriella; Botoman, Lester; Brameld, John M; Salter, Andrew M

In vitro determination of the protein quality of maize varieties cultivated in Malawi using the INFOGEST digestion method. Thumbnail


Authors

Dongfang Li

Gabriella Chiutsi-Phiri

Lester Botoman

Profile image of JOHN BRAMELD

JOHN BRAMELD JOHN.BRAMELD@NOTTINGHAM.AC.UK
Professor of Nutritional Biochemistry

Andrew M Salter



Abstract

There is an urgent need to alleviate protein deficiencies in low-income countries where cereal-based diets dominate. The objective of this study was to use the INFOGEST static digestion method and a recently established analytical workflow to determine the in vitro amino acid digestibility and protein quality of seven maize varieties grown in Malawi. Protein quality was measured using the in vitro digestible indispensable amino acid score (DIAAS). Amino acid digestibility was higher for the dehulled, low fibre, provitamin A maize flour (66%), compared to whole grain maize flours (51-61%), suggesting that the presence of fibre reduced digestibility (p<0.05). Lysine was the limiting amino acid in all varieties, with the following DIAAS values for each variety; Provitamin A maize - 24, SC 719 - 32, Mtsikinya - 37, SC 167 - 39, Quality protein maize (QPM) - 40, Bantum - 40, SC 403 - 44. In addition to the variety of maize, protein quality was dependent on the level of processing and the agronomic practice applied with higher protein quality for the SC 403 variety in which zinc enriched fertilizer was applied. Comparing protein quality data with published in vivo data showed that DIAAS data were in closer agreement than amino acid digestibility data, which was slightly lower than published values, with mean in vitro amino acid digestibilities of 56-70% compared to a mean in vivo value of 77%. Overall, the in vitro method was able to correctly predict both the direction and magnitude of response. The INFOGEST digestion method coupled with the new analytical workflow will therefore be useful in the screening of high protein cereal crops and subsequent development of cereal-based foods with high protein quality. [Abstract copyright: © 2023 The Authors.]

Citation

Muleya, M., Li, D., Chiutsi-Phiri, G., Botoman, L., Brameld, J. M., & Salter, A. M. (2023). In vitro determination of the protein quality of maize varieties cultivated in Malawi using the INFOGEST digestion method. Heliyon, 9(9), Article e19797. https://doi.org/10.1016/j.heliyon.2023.e19797

Journal Article Type Article
Acceptance Date Sep 1, 2023
Online Publication Date Sep 2, 2023
Publication Date 2023-09
Deposit Date Sep 11, 2023
Publicly Available Date Sep 12, 2023
Journal Heliyon
Electronic ISSN 2405-8440
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 9
Issue 9
Article Number e19797
DOI https://doi.org/10.1016/j.heliyon.2023.e19797
Keywords Indispensable amino acids, Lysine, Quality protein maize (QPM), DIAAS, Zinc agronomically biofortified maize, Digestibility
Public URL https://nottingham-repository.worktribe.com/output/25061117
Publisher URL https://www.sciencedirect.com/science/article/pii/S2405844023070056?via%3Dihub#ack0010
Additional Information This article is maintained by: Elsevier; Article Title: In vitro determination of the protein quality of maize varieties cultivated in Malawi using the INFOGEST digestion method; Journal Title: Heliyon; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.heliyon.2023.e19797; Content Type: article; Copyright: © 2023 The Authors. Published by Elsevier Ltd.

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