Transcriptional networks underpinning ploidy related increased leaf potassium in neo-tetraploids

Fischer, Sina; Flis, Paulina; Zhao, Fang-Jie; Salt, David E.

Abstract

Neo-tetraploid Arabidopsis thaliana have elevated leaf potassium (K) driven by processes within the root. The root transcriptome of neo-tetraploids is distinct from diploids, with evidence of altered K homeostasis. Mutational analysis revealed that the canonical K-uptake transporters AKT1 and HAK5 are not required for this elevated leaf K in neo-tetraploids, while the endodermis, root hairs, and SOS signaling are. Contrasting the root transcriptomes of neo-tetraploids and diploids of mutants that block the neo-tetraploid K phenotype, allowed us to identify 91 differentially expressed genes associated with elevated leaf K in neo-tetraploids. This set of genes connects WGD to elevated leaf K, and is enriched in functions such as cell wall and Casparian strip development, and ion-transport, in the endodermis, root hairs, and procambium. This gene set provides tools to test the intriguing idea of recreating the physiological effects of WGD within a diploid genome.

Citation

Fischer, S., Flis, P., Zhao, F.-J., & Salt, D. E. (2021). Transcriptional networks underpinning ploidy related increased leaf potassium in neo-tetraploids

• Working Paper Type: Preprint
• Online Publication Date: Sep 11, 2021
• Publication Date: Sep 11, 2021
• Deposit Date: Mar 17, 2025
• Publicly Available Date: Mar 20, 2025
• DOI: https://doi.org/10.1101/2021.09.09.459632
• Public URL: https://nottingham-repository.worktribe.com/output/24586101
• Publisher URL: https://www.biorxiv.org/content/10.1101/2021.09.09.459632v1
• Additional Information: This article is a preprint and has not been certified by peer review.