Electrochemical Determination of Hydrogen Peroxide and Bicarbonate Using Peroxidase Activity of Copper, Zinc Superoxide Dismutase on Carbon Nanotube, Polypyrrole Nanocomposite Modified Pt Electrode

Balamurugan, Murugesan; Madasamy, Thangamuthu; Pandiaraj, Manickam; Rajkumar, Raju; Rajendran, Ayyapan; Karunakaran, Chandran

Abstract

Measurement of hydrogen peroxide (H2 O2) and bicarbonate (HCO– 3) are essential in human pathology and pathophysiology as it provides valuable information with regard to signalling and prognostic of various diseases viz. oxidative stress and cardiovascular diseases. Therefore, we have developed here a novel electrochemical method for the determination of H2O2and HCO– 3 based on the peroxidase activity of copper, zinc superoxide dismutase (SOD). By immobilizing SOD onto the single walled carbon nanotubes (SWCNT)-polypyrrole (PPy) nanocomposite modified platinum electrode, the concentrations of H2O2and HCO– 3 were measured. The electrochemical behaviour of the SOD modified electrode was examined by cyclic voltammetry exhibiting characteristic quasi-reversible redox peak at the potential,+ 0.06 V versus Ag/AgCl. The peroxidase activity of SOD observed at –0.45 V was linear from 300 nM to 1 mM with a detection limit of 100 nM and sensitivity of 78.6 ± 1.7 nA μ M–1 cm–2. In the presence of HCO– 3, the peroxidase activity of SOD was enhanced linearly with HCO– 3concentration from 500 M to 50 mM. Based on this, we have measured here the HCO– 3 with a detection limit of 200 M and sensitivity of 1.23 μ A mM–1. Further, the concentrations of H2 O2 and HCO– 3 present in the human plasma samples were measured.

• Journal Article Type: Article
• Acceptance Date: Jul 1, 2015
• Online Publication Date: Jul 1, 2015
• Publication Date: Jul 1, 2015
• Deposit Date: Jul 31, 2023
• Journal: Advanced Chemistry Letters
• Print ISSN: 0366-7022
• Publisher: Chemical Society of Japan
• Peer Reviewed: Peer Reviewed
• Volume: 2
• Issue: 1
• Pages: 24-30
• DOI: https://doi.org/10.1166/acl.2015.1052
• Public URL: https://nottingham-repository.worktribe.com/output/23549837
• Publisher URL: https://www.ingentaconnect.com/content/asp/acl/2015/00000002/00000001/art00005