A Volume Miniaturized Hydrogen Peroxide Sensor Based on Peroxidase Activity of Copper(II) Chlorophyllin on Zinc Oxide and Polypyrrole Nanocomposite

Balamurugan, Murugesan; Santharaman, Paulraj; Madasamy, Thangamuthu; Claussen, Jonathan; Karunakaran, Chandran

Abstract

Measurement of hydrogen peroxide (H2O2) is essential in human physiology and pathology as it provides valuable information with regard to signaling and prognostic of various diseases viz. oxidative stress and cardiovascular diseases. Therefore, we developed an alternate volume miniaturized electrochemical enzyme mimetic sensor having high stability, reproducibility and fast response time for the determination of H2O2. The enzyme mimetic sensor was constructed using copper (II) chlorophyllin trisodium (CuCP) functionalized on to the zinc oxide (ZnO)-polypyrrole (PPy) nanocomposite modified screen printed carbon electrode (SPCE). Scanning electron microscope (SEM) was used to characterize the surface morphology of nanocomposite PPy and ZnO-CuCP modified electrodes. The electrochemical behavior of enzyme mimetic sensor was examined by cyclic voltammetry exhibiting a characteristic quasi-reversible peak at the potential, +0.06 V versus Ag/AgCl, for the electrodeposited CuCP. It reveals that the combination of ZnO and CuCP could enhance the sensor performance in terms of sensitivity and selectivity. In addition, new reversible redox peaks at +0.15 and -0.15 V were observed accounting for the mechanism of superoxide anion radical (O2•-) formation. The enzyme mimetic sensor exhibited a linear response over the H2O2 concentration ranges from 300 nM to 1 mM with a detection limit of 100 nM and a sensitivity of 78.4 ± 1.5 nA μM-1 cm-2. Further, the sensor was successfully applied for the determination of H2O2 in biological samples such as human blood and plasma.

Citation

Balamurugan, M., Santharaman, P., Madasamy, T., Claussen, J., & Karunakaran, C. (2016). A Volume Miniaturized Hydrogen Peroxide Sensor Based on Peroxidase Activity of Copper(II) Chlorophyllin on Zinc Oxide and Polypyrrole Nanocomposite. Sensor Letters, 14(7), 665-672. https://doi.org/10.1166/sl.2016.3666

• Journal Article Type: Article
• Online Publication Date: Jul 1, 2016
• Publication Date: Jul 1, 2016
• Deposit Date: Jul 31, 2023
• Journal: Sensor Letters
• Print ISSN: 1546-198X
• Electronic ISSN: 1546-1971
• Publisher: American Scientific Publishers
• Peer Reviewed: Peer Reviewed
• Volume: 14
• Issue: 7
• Pages: 665-672
• DOI: https://doi.org/10.1166/sl.2016.3666
• Public URL: https://nottingham-repository.worktribe.com/output/23549831
• Publisher URL: https://www.ingentaconnect.com/content/asp/senlet/2016/00000014/00000007/art00003;jsessionid=2ntsonl6rbjpq.x-ic-live-01