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PUCHI regulates very long chain fatty acid biosynthesis during lateral root and callus formation

Trinh, Duy-Chi; Lavenus, Julien; Goh, Tatsuaki; Boutté, Yohann; Drogue, Quentin; Vaissayre, Virginie; Tellier, Frédérique; Lucas, Mikaël; Voß, Ute; Gantet, Pascal; Faure, Jean-Denis; Dussert, Stéphane; Fukaki, Hidehiro; Bennett, Malcolm J.; Laplaze, Laurent; Guyomarc’h, Soazig

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Authors

Duy-Chi Trinh

Julien Lavenus

Tatsuaki Goh

Yohann Boutté

Quentin Drogue

Virginie Vaissayre

Frédérique Tellier

Mikaël Lucas

UTE VOSS ute.voss@nottingham.ac.uk
Assistant Professor

Pascal Gantet

Jean-Denis Faure

Stéphane Dussert

Hidehiro Fukaki

Laurent Laplaze

Soazig Guyomarc’h



Abstract

© 2019 National Academy of Sciences. All rights reserved. Lateral root organogenesis plays an essential role in elaborating plant root system architecture. In Arabidopsis, the AP2 family transcription factor PUCHI controls cell proliferation in lateral root primordia. To identify potential targets of PUCHI, we analyzed a time course transcriptomic dataset of lateral root formation. We report that multiple genes coding for very long chain fatty acid (VLCFA) biosynthesis enzymes are induced during lateral root development in a PUCHI-dependent manner. Significantly, several mutants perturbed in VLCFA biosynthesis show similar lateral root developmental defects as puchi-1. Moreover, puchi-1 roots display the same disorganized callus formation phenotype as VLCFA biosynthesis-deficient mutants when grown on auxin-rich callus-inducing medium. Lipidomic profiling of puchi-1 roots revealed reduced VLCFA content compared with WT. We conclude that PUCHI-regulated VLCFA biosynthesis is part of a pathway controlling cell proliferation during lateral root and callus formation.

Citation

Trinh, D., Lavenus, J., Goh, T., Boutté, Y., Drogue, Q., Vaissayre, V., …Guyomarc’h, S. (2019). PUCHI regulates very long chain fatty acid biosynthesis during lateral root and callus formation. Proceedings of the National Academy of Sciences, 116(28), 14325-14330. https://doi.org/10.1073/pnas.1906300116

Journal Article Type Article
Acceptance Date Jun 4, 2019
Online Publication Date Jun 24, 2019
Publication Date Jun 24, 2019
Deposit Date Jun 28, 2019
Publicly Available Date Jul 1, 2019
Journal Proceedings of the National Academy of Sciences
Print ISSN 0027-8424
Electronic ISSN 1091-6490
Publisher National Academy of Sciences
Peer Reviewed Peer Reviewed
Volume 116
Issue 28
Pages 14325-14330
DOI https://doi.org/10.1073/pnas.1906300116
Keywords Multidisciplinary
Public URL https://nottingham-repository.worktribe.com/output/2230912
Publisher URL https://www.pnas.org/content/early/2019/06/20/1906300116
Contract Date Jun 28, 2019

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