Morphology of migrating telocytes and their potential role in stem cell differentiation during cartilage development in catfish (Clarias gariepinus)

Abd-Elhafeez, Hanan H.; Rutland, Catrin Sian; Soliman, Soha A.

Abstract

Telocytes (TCs) are present in a broad range of species and regulate processes including homeostasis, tissue regeneration and immunosurveillance. This novel study describes the morphological features of migrating TCs and their role during cartilage development within the air-breathing organ in Clarias gariepinus, the African sharptooth catfish. Light microscopy (LM), transmission electron microscopy (TEM), and immunohistochemistry (IHC) were used to examine the TCs. TCs had a cell body and telopodes which formed 3D networks in the cartilage canals and extended their telopodes to become the foremost cellular elements penetrating the cartilage matrix. The TCs were also rich in lysosomes that secreted products to the extracellular matrix (ECM). In addition, TCs formed a homocellular synaptic-like structure that had a synaptic cleft, and the presynaptic portion consisted of a slightly expanded terminal of the telopodes which contained intermediate filaments and secretory vesicles. Gap junctions were also identified between TCs, which also connected to mesenchymal stem cells, differentiating chondrogenic cells, macrophages, apoptotic cells, and endothelial cells. In addition to describing the basic morphology of TCs, the current study also investigated migrating TCs. The TC telopodes acquired an irregular contour when migrating rather than exhibiting an extended profile. Migrating TCs additionally had ill-defined cell bodies, condensed chromatin, thickened telopodes, and podoms which were closely attached to the cell body. The TCs also expressed markers for MMP-9, CD117, CD34 and RhoA. In conclusion, TCs may play multiple roles during development and maturation, including promoting angiogenesis, cell migration, and regulating stem cell differentiation.
Research Highlights

Clarias gariepinus telocytes form 3D networks, extend their telopodes and contain lysosomes.
Telocytes form a homocellular synaptic-like structure including clefts and a slightly expanded terminal of the telopodes which contains intermediate filaments and secretory vesicles.
Gap junctions form between telocytes, which also connect to mesenchymal stem cells, differentiating chondrogenic cells, macrophages, apoptotic cells, and endothelial cells.
Migrating telocytes were discovered which had ill-defined cell bodies, condensed chromatin, thickened telopodes exhibiting irregular contours, and podoms which were closely attached to the cell body.

Citation

Abd-Elhafeez, H. H., Rutland, C. S., & Soliman, S. A. (2023). Morphology of migrating telocytes and their potential role in stem cell differentiation during cartilage development in catfish (Clarias gariepinus). Microscopy Research and Technique, 86(9), 1108-1121. https://doi.org/10.1002/jemt.24374

• Journal Article Type: Article
• Acceptance Date: Jun 8, 2023
• Online Publication Date: Jun 20, 2023
• Publication Date: 2023-09
• Deposit Date: Jun 22, 2023
• Publicly Available Date: Jun 22, 2023
• Journal: Microscopy Research and Technique
• Print ISSN: 1059-910X
• Electronic ISSN: 1097-0029
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 86
• Issue: 9
• Pages: 1108-1121
• DOI: https://doi.org/10.1002/jemt.24374
• Keywords: air-breathing organ, cartilage development, Clarias gariepinus, mesenchymal stem cells, migrating telocytes
• Public URL: https://nottingham-repository.worktribe.com/output/22181196
• Publisher URL: https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jemt.24374