Ana Belén Carrillo-Galvez
Mesenchymal stromal cells express GARP/LRRC32 on their surface: Effects on their biology and immunomodulatory capacity
Carrillo-Galvez, Ana Belén; Cobo, Marién; Cuevas-Ocaña, Sara; Gutiérrez-Guerrero, Alejandra; Sánchez-Gilabert, Almudena; Bongarzone, Pierpaolo; García-Pérez, Angélica; Muñoz, Pilar; Benabdellah, Karim; Toscano, Miguel G.; Martín, Francisco; Anderson, Per
Authors
Marién Cobo
Sara Cuevas-Ocaña
Alejandra Gutiérrez-Guerrero
Almudena Sánchez-Gilabert
Pierpaolo Bongarzone
Angélica García-Pérez
Pilar Muñoz
Karim Benabdellah
Miguel G. Toscano
Francisco Martín
Per Anderson
Contributors
Dr SARA CUEVAS OCANA SARA.CUEVASOCANA@NOTTINGHAM.AC.UK
Researcher
Abstract
Mesenchymal stromal cells (MSCs) represent a promising tool for therapy in regenerative medicine, transplantation, and autoimmune disease due to their trophic and immunomodulatory activities. However, we are still far from understanding the mechanisms of action of MSCs in these processes. Transforming growth factor (TGF)-β1 is a pleiotropic cytokine involved in MSC migration, differentiation, and immunomodulation. Recently, glycoprotein A repetitions predominant (GARP) was shown to bind latency-associated peptide (LAP)/TGF-β1 to the cell surface of activated Foxp3<sup>+</sup> regulatory T cells (Tregs) and megakaryocytes/platelets. In this manuscript, we show that human and mouse MSCs express GARP which presents LAP/TGF-β1 on their cell surface. Silencing GARP expression in MSCs increased their secretion and activation of TGF-β1 and reduced their proliferative capacity in a TGF-β1-independent manner. Importantly, we showed that GARP expression on MSCs contributed to their ability to inhibit T-cell responses in vitro. In summary, we have found that GARP is an essential molecule for MSC biology, regulating their immunomodulatory and proliferative activities. We envision GARP as a new target for improving the therapeutic efficacy of MSCs and also as a novel MSC marker. Stem Cells 2015;33:183-195
Citation
Carrillo-Galvez, A. B., Cobo, M., Cuevas-Ocaña, S., Gutiérrez-Guerrero, A., Sánchez-Gilabert, A., Bongarzone, P., …Anderson, P. (2015). Mesenchymal stromal cells express GARP/LRRC32 on their surface: Effects on their biology and immunomodulatory capacity. STEM CELLS, 33(1), 183-195. https://doi.org/10.1002/stem.1821
Journal Article Type | Article |
---|---|
Acceptance Date | Aug 10, 2014 |
Online Publication Date | Dec 18, 2014 |
Publication Date | Jan 1, 2015 |
Deposit Date | Jun 16, 2023 |
Publicly Available Date | Jun 23, 2023 |
Journal | Stem Cells |
Print ISSN | 1066-5099 |
Electronic ISSN | 1549-4918 |
Publisher | AlphaMed Press |
Peer Reviewed | Peer Reviewed |
Volume | 33 |
Issue | 1 |
Pages | 183-195 |
DOI | https://doi.org/10.1002/stem.1821 |
Public URL | https://nottingham-repository.worktribe.com/output/21926820 |
Publisher URL | https://academic.oup.com/stmcls/article/33/1/183/6407053?login=false |
Files
stmcls_33_1_183
(697 Kb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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