Listeria innocua Dps as a nanoplatform for bioluminescence based photodynamic therapy utilizing Gaussia princeps luciferase and zinc protoporphyrin IX

Al-Ani, Ali W.; Zhang, Lei; Ferreira, Lenny; Turyanska, Lyudmila; Bradshaw, Tracey D.; Thomas, Neil R.

Abstract

Listeria innocua DNA binding protein from starved cells (LiDps) belongs to the ferritin family and provides a promising self-assembling spherical 12-mer protein scaffold for the generation of functional nanomaterials. We report the creation of a Gaussia princeps luciferase (Gluc)-LiDps fusion protein, with chemical conjugation of Zinc (II)-protoporphyrin IX (ZnPP) to lysine residues on the fusion protein (giving Gluc-LiDps-ZnPP). The Gluc-LiDps-ZnPP conjugate is shown to generate reactive oxygen species (ROS) via Bioluminescence Resonance Energy Transfer (BRET) between the Gluc (470-490 nm) and ZnPP. In vitro, Gluc-LiDps-ZnPP is efficiently taken up by tumorigenic cells (SKBR3 and MDA-MB-231 breast cancer cells). In the presence of coelenterazine, this construct inhibits the proliferation of SKBR3 due to elevated ROS levels. Following exposure to Gluc-LiDps-ZnPP, migration of surviving SKBR3 cells is significantly suppressed. These results demonstrate the potential of the Gluc-LiDps-ZnPP conjugate as a platform for future development of an anticancer photodynamic therapy agent.

Citation

Al-Ani, A. W., Zhang, L., Ferreira, L., Turyanska, L., Bradshaw, T. D., & Thomas, N. R. (2019). Listeria innocua Dps as a nanoplatform for bioluminescence based photodynamic therapy utilizing Gaussia princeps luciferase and zinc protoporphyrin IX. Nanomedicine: Nanotechnology, Biology and Medicine, 20, Article 102005. https://doi.org/10.1016/j.nano.2019.04.008

• Journal Article Type: Article
• Acceptance Date: Apr 18, 2019
• Online Publication Date: Apr 29, 2019
• Publication Date: Aug 31, 2019
• Deposit Date: Jun 12, 2019
• Publicly Available Date: Jun 12, 2019
• Journal: Nanomedicine: Nanotechnology, Biology and Medicine
• Print ISSN: 1549-9634
• Electronic ISSN: 1549-9642
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 20
• Article Number: 102005
• DOI: https://doi.org/10.1016/j.nano.2019.04.008
• Keywords: Molecular Medicine; General Materials Science; Medicine (miscellaneous); Bioengineering; Pharmaceutical Science; Biomedical Engineering
• Public URL: https://nottingham-repository.worktribe.com/output/2178664
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S1549963419300899?via%3Dihub
• Contract Date: Jun 12, 2019