Ali W. Al-Ani
Listeria innocua Dps as a nanoplatform for bioluminescence based photodynamic therapy utilizing Gaussia princeps luciferase and zinc protoporphyrin IX
Al-Ani, Ali W.; Zhang, Lei; Ferreira, Lenny; Turyanska, Lyudmila; Bradshaw, Tracey D.; Thomas, Neil R.
Tracey D. Bradshaw
Neil R. Thomas
Listeria innocua DNA binding protein from starved cells (LiDps) belongs to the ferritin family and provides a promising self-assembling spherical 12-mer protein scaffold for the generation of functional nanomaterials. We report the creation of a Gaussia princeps luciferase (Gluc)-LiDps fusion protein, with chemical conjugation of Zinc (II)-protoporphyrin IX (ZnPP) to lysine residues on the fusion protein (giving Gluc-LiDps-ZnPP). The Gluc-LiDps-ZnPP conjugate is shown to generate reactive oxygen species (ROS) via Bioluminescence Resonance Energy Transfer (BRET) between the Gluc (470-490 nm) and ZnPP. In vitro, Gluc-LiDps-ZnPP is efficiently taken up by tumorigenic cells (SKBR3 and MDA-MB-231 breast cancer cells). In the presence of coelenterazine, this construct inhibits the proliferation of SKBR3 due to elevated ROS levels. Following exposure to Gluc-LiDps-ZnPP, migration of surviving SKBR3 cells is significantly suppressed. These results demonstrate the potential of the Gluc-LiDps-ZnPP conjugate as a platform for future development of an anticancer photodynamic therapy agent.
|Journal Article Type||Article|
|Publication Date||Aug 31, 2019|
|Journal||Nanomedicine: Nanotechnology, Biology and Medicine|
|Peer Reviewed||Peer Reviewed|
|APA6 Citation||Al-Ani, A. W., Zhang, L., Ferreira, L., Turyanska, L., Bradshaw, T. D., & Thomas, N. R. (2019). Listeria innocua Dps as a nanoplatform for bioluminescence based photodynamic therapy utilizing Gaussia princeps luciferase and zinc protoporphyrin IX. Nanomedicine: Nanotechnology, Biology and Medicine, 20, doi:10.1016/j.nano.2019.04.008|
|Keywords||Molecular Medicine; General Materials Science; Medicine (miscellaneous); Bioengineering; Pharmaceutical Science; Biomedical Engineering|
Listeria innocua Dps as a nanoplatform for bioluminescence based photodynamic therapy utilizing Gaussia princeps luciferase and zincprotoporphyrin IX
You might also like
In search of effective therapies to overcome resistance to Temozolomide in brain tumours
Realization of Universal Quantum Gates with Spin-Qudits in Colloidal Quantum Dots