Global serum proteomic changes in water buffaloes infected with Fasciola gigantica

Zhang, Fu-Kai; Hu, Rui-Si; Elsheikha, Hany M.; Sheng, Zhao-An; Zhang, Wei-Yu; Zheng, Wen-Bin; Zhu, Xing-Quan; He, Jun-Jun

Abstract

Background: The liver fluke Fasciola gigantica modulates several signaling pathways in infected buffaloes to facilitate its survival and establishment of persistent infection. In response to the parasite invasion, buffaloes activate innate and adaptive immune responses to counter the parasite infection. To detect new proteins that might be involved in the interaction between F. gigantica and the buffaloes, and that also might serve as biomarkers for fasciolosis, we used proteomic techniques to study the serum proteome of buffaloes during F. gigantica infection. Here, we used an isobaric tags for relative and absolute quantitation (iTRAQ)‑based quantitative proteomic approach to identify serum proteins that are differentially expressed in infected buffaloes compared to uninfected control buffaloes. Additionally, we applied a parallel reaction monitoring (PRM) assay to validate specific proteins identified by the iTRAQ method. Results: A total of 313, 459 and 399 proteins were identified at 3, 42 and 70 days post‑infection, respectively; of these 92, 93 and 138 were differentially abundant proteins. Some of the identified differentially abundant proteins, includ‑ ing complement factor H related 5, complement component C6, complement component C7, amine oxidase, plasma serine protease inhibitor and lysozyme, are known to be involved in complement system activation, blood coagula‑ tion, platelet activation, lymphocyte’s adhesion and lysozyme hydrolysis. Analysis of data for all three time points after infection identified six significantly upregulated proteins in infected serum that separated infected and uninfected buffaloes into distinct clusters. Further PRM analysis confirmed the expression of five proteins, namely MHC class I antigen, Beta‑2‑microglobulin, NID2 protein, Fetuin‑B and Fibrinogen gamma‑B chain. Conclusions: These findings provide novel insights into the serum proteomics signature of buffaloes during F. gigantica infection.

Citation

Zhang, F.-K., Hu, R.-S., Elsheikha, H. M., Sheng, Z.-A., Zhang, W.-Y., Zheng, W.-B., …He, J.-J. (2019). Global serum proteomic changes in water buffaloes infected with Fasciola gigantica. Parasites and Vectors, 12(1), Article 281. https://doi.org/10.1186/s13071-019-3533-5

• Journal Article Type: Article
• Acceptance Date: May 27, 2019
• Online Publication Date: Jun 3, 2019
• Publication Date: Jun 3, 2019
• Deposit Date: Jun 7, 2019
• Publicly Available Date: Jun 7, 2019
• Journal: Parasites & Vectors
• Electronic ISSN: 1756-3305
• Publisher: Springer Nature
• Peer Reviewed: Peer Reviewed
• Volume: 12
• Issue: 1
• Article Number: 281
• DOI: https://doi.org/10.1186/s13071-019-3533-5
• Keywords: Parasitology; Infectious Diseases
• Public URL: https://nottingham-repository.worktribe.com/output/2157777
• Publisher URL: https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-019-3533-5
• Contract Date: Jun 7, 2019