Development of a New A –Genome-Specific Single Nucleotide Polymorphism Marker Set for the Molecular Characterization of Wheat– Introgression Lines

Cseh, Andras; Megyeri, Maria; Hubbart-Edwards, Stella; Yang, Caiyun; Scholefield, Duncan; Ashling, Stephen S.; King, Ian P.; King, Julie; Grewal, Surbhi

Abstract

© 2019 The Author(s). Cultivated einkorn wheat (Triticum monococcum L. subsp. monococcum, 2n = 2x = 14, AmAm) and its wild relative T. monococcum subsp. aegilopoides are important sources of economically useful genes that can be exploited for wheat (Triticum aestivum L.) breeding. Einkorn has excellent resistance to fungal diseases and gene transfer is relatively simple via standard breeding methods. To fulfill the growing demand by modern prebreeding programs for a cost-effective high-throughpuprocedure for accurately detecting introgressed chromosomes or chromosome segments from T. monococcum into wheat, we used the Axiom Wheat-Relative Genotyping Array and developed a set of Am genome-specific exome-based single nucleotide polymorphism (SNP) markers suitable for rapid identification of T. monococcum chromatin in a wheat background. We identified 1247 polymorphic SNPs between T. monococcum and wheat. We identified 191 markers across all seven chromosomes of T. monococcum that are also present on an existing Triticum urartu Thum. ex Gandil. genetic map and potentially ordered them on the basis of the high macrocollinearity and conservation of marker order between T. monococcum and T. urartu. The marker set has been tested on leaf-rust-resistant BC3 F4 progenies of wheat–T. monococcum hybrids. Two markers (AX-94492165, AX-95073542) placed on the distal end of the chromosome arm 7AL detected a T. monococcum introgression into wheat. The SNP marker set thus proved highly effective in the identification of T. monococcum chromatin in a wheat background, offering a reliable method for screening and selecting wheat–T. monococcum introgression lines, a procedure that could significantly speed up prebreeding programs.

Citation

Cseh, A., Megyeri, M., Hubbart-Edwards, S., Yang, C., Scholefield, D., Ashling, S. S., …Grewal, S. (2019). Development of a New A –Genome-Specific Single Nucleotide Polymorphism Marker Set for the Molecular Characterization of Wheat– Introgression Lines. Plant Genome, The, 12(3), 1-7. https://doi.org/10.3835/plantgenome2018.12.0098

• Journal Article Type: Article
• Acceptance Date: Apr 24, 2019
• Online Publication Date: Jul 11, 2019
• Publication Date: 2019-11
• Deposit Date: May 9, 2019
• Publicly Available Date: Aug 14, 2019
• Journal: The Plant Genome
• Electronic ISSN: 1940-3372
• Publisher: Crop Science Society of America
• Peer Reviewed: Peer Reviewed
• Volume: 12
• Issue: 3
• Pages: 1-7
• DOI: https://doi.org/10.3835/plantgenome2018.12.0098
• Keywords: Agronomy and Crop Science; Plant Science; Genetics
• Public URL: https://nottingham-repository.worktribe.com/output/2034170
• Publisher URL: https://dl.sciencesocieties.org/publications/tpg/abstracts/0/0/180098
• Contract Date: May 9, 2019